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Unformatted text preview: Enzymes to know: Chapter 7 (DNA replication): • Pancreatic deoxyribonuclease* – specifically degrades DNA but does not degrade protein • Topoisomerases* – change the LK as well as untangle DNA molecules. Function by producing single-stranded (type I) or double-stranded (type II) breaks in the sugar-phosphate backbone of the DNA. • Gyrase* – type II topoisomerase responsible for the negative supercoils in prokaryotic chromosomes • Pyrophosphatase* – catalyzes the hydrolysis of a pyrophosphate into two phosphate groups • Helicase (DnaB) – breaks H bonds btwn bases to open the replication fork. Requires ATP . • DNA polymerase III* – primary enzyme involved in the replication of the chromosome. Initiates DNA synthesis immediately after the primer is laid down. • Exonuclease – degrades a nucleic acid polymer, but must begin at the end of the nucleic acid. Will be either 5’-3’ or 3’-5’ • Endonuclease – also degrades nucleic acid polymer but can start at the middle of the break (ex. Restriction enzymes) • RNAseH* – removes all of the primer except the ribonucleotide directly linked to...
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