genChap5b - Chapter5b(Draft) GeneticsofDNAFunction...

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Chapter 5b  (Draft) Genetics of DNA Function Do the odd problems. Recall the “Big picture.” Biosynthetic pathways. Turn to page 269. If we add the blocked compound or any other later compound the mutant will  grow. Compounds found later in the pathway will support the growth of the most  mutants. From the table on page 269: The order of compounds is:  E, A, C, B, D, G. At which point in the pathway is each mutant blocked? A mutant between E and A cannot be supported by compound E but it can be by  all the others. Mutant 5 must be blocked in the E-A conversion. The “one-gene-one-enzyme hypothesis: Each locus codes for one protein enzyme. George Beadle Edward Tatum Nobel Prize Since falsified Provide two observations that falsify this theory. Used  Neurospora  (The pink bread mold.) Technique p 270 Irradiated spores. Place in minimal medium 
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Filter out wild type Plate mutants on complete medium Collect progeny spores  Plate progeny spores on many media to determine deficiency Focused on mutant strains deficient in the production of arg, arginine. Precursor  > enzyme X >  ornithine  > enzyme Y >  citrulline  > enzyme Z >  arginine .
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genChap5b - Chapter5b(Draft) GeneticsofDNAFunction...

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