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experiment 3 - Samuel De Barry 76252790 Partner:Simon Le...

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Samuel De Barry 76252790 Partner:Simon Le October 27, 2009 Lab 14 Section 40443 Experiment 4: Thin-Layer Chromatography Thin-layer chromatography is a technique that is commonly used to identify organic compounds. Using thin-layer chromatography allows someone to determine if two compounds are identical, the progress of the reaction as well as the total number of compounds within a mixture. The purpose of this lab was to use thin-layer chromatography to identify an unknown substance as well as determine the purity of caffeine extracted from tea. In the lab, first, five known analgesics were analyzed using thin layer chromatography to determine their R f values relative to ibuprofen. . After the whole extraction process was complete, .055 g of caffeine was recovered from 3.502g of tea leaves. The tea was brewed in water and calcium carbonate. It was brewed in water because caffeine is soluble in water so it allowed us to extract the caffeine from the tea.
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