2008 M2 - 1 BIS 101 - 002 MIDTERM II May 16, 2008 READ THE...

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1 BIS 101 - 002 MIDTERM II May 16, 2008 READ THE FOLLOWING INSTRUCTIONS: Write your name legibly on each page of this exam. All work must be done in ink . Show all your work. Points will be subtracted for incorrect answers in multiple-choice questions. However, you will not get a negative total score for such a question. This exam is worth 100 points. Bonus question is optional and is not part of the 100 points. You have 50 min. to complete the exam. Authorization for public distribution of graded examination I, ____________________________________, ID _________________________ authorize the University to publicly distribute my grated examination for BIS 101-003. Date_______________________________ Signed: _____________________________ Good Luck!
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2 Name:______________________________ P a g e : 3 ________ 4 ________ 5 ________ 6 ________ 7 ________ 8 ________ Total ________ Bonus_________
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3 Name:______________________________ (1) In the fungus Neurospora, a strain that was auxotrophic for thiamine (mutant allele t ) was crossed to a strain that was auxotrophic for methionine (mutant allele m ). A total of 400 linear, ordered asci were isolated and classified into the following groups. Spore pair The number of asci of indicated type 1 and 2 t + t + t + t + t m t m 3 and 4 t + t m + m + + t m + + 5 and 6 + m + + t + t m + + t + 7 and 8 + m + m + m + m + + + m 260 76 4 54 1 5 PD T PD T NPD T (a) Indicate in each column if the ascus was T, NPD or PD. (4 points) (b) Select Perkins’ formula from the formulas below for the computation of distance in map units (cM) and calculate the distance in map units between t and m. (4 points) map units = 50 (T + 6NPD) map units = 100 (0.5T +NPD) map units = No. recombined chromosomes/ total chromosomes map units = No. recombined chromosomes/ 0.5total asci map units = No T + NPD/ 0.5 total asci f(T) = 135/400 = 0.338 f(NPD) = 1/400 = 0.0025 cM = 50 (0.338 + 6 x 0.0025) = 17.6 (2) E. coli DNA polymerase I has the following properties. Circle all correct answers. (6 points)
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This note was uploaded on 11/14/2009 for the course NPB 33042 taught by Professor Carstens during the Spring '09 term at UC Davis.

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2008 M2 - 1 BIS 101 - 002 MIDTERM II May 16, 2008 READ THE...

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