Study Guide Chapters 4 5 - What are the different types of...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Review Sheet DNA, RNA Introduction Explain the structural differences between DNA and RNA in chemical terms. Describe the main features of the double helical structure of DNA. What are the forces that contribute to the stability of the double helix? Describe the experimental evidence supporting the semi-conservative replication of DNA. Explain the terms Tm, annealing and hyperchromic effect. What are the differences in the re- association of melted DNA strands during slow cooling as opposed to sudden cooling? Explain the reaction catalyzed by DNA polymerase 1 including the mechanism of chain elongation, direction of synthesis, co-factor requirements, primer and nature of the template requirements. What are the different types of RNAs and their relative abundance in E.coli ? What is the function of each type of RNA? Write the reaction catalyzed by RNA polymerase including the requirements of the enzyme.
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: What are the different types of promoters found in eukaryotic and prokaryotic gene? What function do they serve? What are Introns and Exons? What are the main features of the genetic code? Explain how restriction endonucleases cleave the substrate DNA. Explain Southern blotting and Northern blotting techniques. What is the principle behind DNA sequencing by dideoxy chain termination technique? What are the reagents needed for DNA sequencing? Explain the principle involved in DNA cloning. What is the purpose of the vector? What is the principle underlying PCR technique for DNA amplification? How does siRNA silence the expression of a specific RNA molecule? Explain the basis of oligonucleotide mediated site-directed mutagenesis. What can DNA recombination be used for?...
View Full Document

This note was uploaded on 11/14/2009 for the course BIOLCHEM 415 taught by Professor Michaeluhler during the Winter '06 term at University of Michigan.

Ask a homework question - tutors are online