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Unformatted text preview: What are the different types of promoters found in eukaryotic and prokaryotic gene? What function do they serve? What are Introns and Exons? What are the main features of the genetic code? Explain how restriction endonucleases cleave the substrate DNA. Explain Southern blotting and Northern blotting techniques. What is the principle behind DNA sequencing by dideoxy chain termination technique? What are the reagents needed for DNA sequencing? Explain the principle involved in DNA cloning. What is the purpose of the vector? What is the principle underlying PCR technique for DNA amplification? How does siRNA silence the expression of a specific RNA molecule? Explain the basis of oligonucleotide mediated site-directed mutagenesis. What can DNA recombination be used for?...
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This note was uploaded on 11/14/2009 for the course BIOLCHEM 415 taught by Professor Michaeluhler during the Winter '06 term at University of Michigan.
- Winter '06