Study Guide Chapters 29 30

Study Guide Chapters 29 30 - What are the different steps...

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RNA Synthesis and Splicing - Chapter 29 Study Questions What are the different types of promoters that are required for the transcription of genes in prokaryotic and eukaryotic cells? What are the different components of E-coli RNA Polymerase? What is the function of Sigma (σ) subunit in transcription? Describe the processes involved in initiation, elongation and termination of transcription in prokaryotic and eukaryotic cells. What is a transcription bubble? What are the different types of RNA Polymerases found in a eukaryotic cell? How do they differ in function? What are processes involved in the maturation of rRNA and tRNA in a prokaryotic and a eukaryotic cell? Why is RNA splicing necessary in a eukaryotic cell? What is a splicesome? What is the biochemical (structural) mechanism of splicesome-mediated intron splicing? What is a catalytic RNA? What is the evidence for its existence? Protein Synthesis - Chapter 30
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Unformatted text preview: What are the different steps (reactions) that lead to the attachment of an amino acid at the CCA terminus of a tRNA molecule? What are anticodons? Explain the degeneracy of the genetic code on the basis of how a single tRNA molecule is able to recognize multiple codons that specify a single amino acid. What are the steps involved in the initiation of protein synthesis? How does it differ in eukaryotic and prokaryotic cells? Describe the reactions involved in peptide bond formation and elongation of protein synthesis. How is protein synthesis terminated? Explain how streptomycin, tetracychin and erythromycin inhibit protein synthesis? What is a signal peptide? How does a nascent protein containing a signal peptide sequence traverse to the lumen of the endoplasmic reticulum? How does sorting of proteins destined for plasma membrane localization or secretion occur in a eukaryotic cell?...
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