Lab Report 3 - Metabolism in Goldfish - Material and Methods

Lab Report 3 - Metabolism in Goldfish - Material and...

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u ll Hypothesis: Darkness will not decrease the metabolic rate of the gold fish. START END Clean up. Collect 4 fish. (Wash hands.) Return fish. Weigh 1 st 2 fish. Record data. Place 1 st 2 fish in chamber. Collect [O 2 ] every 20 sec for 10 min. Replace fish water. Put on cover, let fish adjust 5 min. Run 60 sec trial. Graph in Excel. Collect [O 2 ] every 20 sec for 10 min. Let fish adjust 5 min. Run 60 sec trial. Repeat light/dark measurements as before. Weigh next 2 fish. Graph in Excel. Materials and Methods Before beginning the experiment, a flow chart (attached) was created to anticipate and resolve any possible problems. To begin, four fish were collected and put into 200mL of fish water. Then they were separated into two groups, group A and group B. Both groups were weighed, and group A was placed into the oxygen chamber with an additional 200mL of water. Fish were allowed to adjust for five minutes. A sixty second trial of LoggerLite was run on the fish. LoggerLite uses an oxygen probe to assess oxygen concentration in mg/L over time –
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Unformatted text preview: collected every twenty seconds. Next the full ten minute run of LoggerLite was performed on the fish. Raw data was recorded and put into an Excel sheet, generating a graph and an equation for the slope of the line. Next, the 400mL of deoxygenated water was replaced with 400mL of fresh water and the chamber was covered with a dark cloth. Again, the fish were allowed to acclimate for five minutes, a thirty second trial was performed, and the full ten minute test was performed. This same procedure was then duplicated with group B. The slopes of the oxygen intake between the control and experimental groups were analyzed to see if there was a statistical difference between the two. From the data, a mean and p-value were determined. Lastly, a t-test is performed to determine if there was a statistical difference between metabolism performed in light and metabolism performed in darkness. Clean up....
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Lab Report 3 - Metabolism in Goldfish - Material and...

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