Replication2-Genetic Code1-actual

Replication2-Genetic Code1-actual - Power point slides

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Foundations of Biological Science I (BioSci 150) C ResponseWare Users Web Address: http://www.r wpoll.com/ Session ID: 469409 Clicker Users Please key in the following on your TurningPoint Clickers: ‘GO-7-3-GO’ Look for the sustained green LED after Pressing GO the second time. You may need to repeat the GO-#-#-GO cycle a few times to get the stained green light. Troubleshooting: If you are not getting the Correct # of points in the Gradebook for clicker participation, 1. make sure your clicker is correctly registered. 2. Program your clicker again; use it correctly. 1. pull the battery tabs or put new batteries in your clicker. 2. Replaced your clicker? register the new one. 3. If all else fails – go to the Help Desk, Bol 225 1. Go to rwpoll.com. 2. Click My Account. 3. 4. Enter the Session ID below. 5. Click Join. Troubleshooting: 1. No/lost connection: a) b) if you have the option, switch from wireless to data plan or vice-versa. c) Change seats in the classroom to get a better connection. 2. Slow response: switch to wireless connection
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Foundations of Biological Science I (BioSci 150) C Structure of DNA- a reminder: 3’ 5’ Making a phosphodiester linkage between the 3’ C on deoxyribose of one nucleotide to the 5’ C nucleotide of a second nucleotide during DNA synthesis creates a molecule with a 5’ end and a 3’ end :
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Foundations of Biological Science I (BioSci 150) C When Watson & Crick built their model of the double helix, they found that they got good H- bonding between the two strands of DNA only if they were antipaerallel – i.e., with the 5’ end of one strand opposite the 3’ end of the other, complementary strand.
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Foundations of Biological Science I (BioSci 150) C DNA Replication Cont’d Have seen: 1. how DNA was confirmed as the genetic material 2. how the structure of the double helix was worked out 3. that DNA polymerases catalyze template-dependent replication 4. that replication is semi-conservative Next up: The biochemical and geographical details of replication
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Foundations of Biological Science I (BioSci 150) C In E. coli , DNA polymerases II & III, are faster, better catalysts than A. Kornberg’s DNA polymerase I… The fastest is DNA polymerase III , the main enzyme of replication.
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Foundations of Biological Science I (BioSci 150) C Properties of DNA polymerases Template-dependent 5’ - 3’ elongation Can only add to an existing strand of nucleic acid 3’ 3’ 5’ Parental (template) strand 5’ 3’ dNTPs
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Replication2-Genetic Code1-actual - Power point slides

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