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Unformatted text preview: several solutions of Fe(NO3)3 of various concentrations, to which thiocyanate was added, were measuered by spectrophotometry in order to plot a standard curve. From the standard curve the molar absorbtion constant for the complex of Fe(SCN)2+. Saliva was then taken and centrifuged at a rate of 6000 rpm for 5 minutes. 500 microliters of saliva were then transferred to a 25 ml volumetric flask. Fe(NO3)3 was then added to form the red complex of Fe(SCN)2+. The complex’s absorbance was the measured from which the concentration present in the saliva could then be measured....
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This note was uploaded on 11/22/2009 for the course CLAS 170 taught by Professor Staley during the Spring '07 term at Maryland.
- Spring '07