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Unformatted text preview: measured. The absorbance measurement took the absorbance value of NADH, the product of NAD+ accepting hydrogen. This value used to take the equilibrium concentration of NADH by using the molar absorptivity value for NADPH found by creating a standard curve. The concentration of NADH was found to be 9.1E-5 M, which was also the concentration of acetaldehyde as well as the change in the initial concentration of ethanol and NAD+. From this and the equation for the reaction, the equilibrium constant K was calculated. It turns out that the calculated K value for the experiment performed by my group was about .007. What this shows is that alcohol is converted to acetaldehyde but amount converted is not that great. In the real world, this means that it takes a decent amount of NAD+ and alcohol dehydrogenase to detoxify oneself from a night of drinking. This could in fact be the reason why it takes a long time for people to sober up when very intoxicated....
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This note was uploaded on 11/22/2009 for the course CLAS 170 taught by Professor Staley during the Spring '07 term at Maryland.
- Spring '07