abstract for experiment 3

abstract for experiment 3 - measured The absorbance...

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Jon Bickerstaff CHEM 277 Experiment 3 Alcohol Dehydrogenase and Equilibrium Constants When people ingest alcohol most do not realize what is actually going on in their bodies. Alcohol is toxic to human cells and must be processed by the liver to prevent toxicity. The process is performed by removing hydrogen from ethanol and using a electron acceptor, in this case NAD+. They only way this is achieved are with the catalytic enzyme alcohol dehydrogenase. This reaction has an equilibrium constant associated with it which shows how much alcohol in the system is converted to its less harmful product acetaldehyde. The equilibrium constant for this reaction was calculated by performing a reaction of alcohol and NAD+ with the presence of alcohol dehydrogenase. 800 ml of both NAD+ at .29mM and ethanol at 45mM were mixed to which 100 ml of alcohol dehydrogenase. This reaction was let run until the system was at equilibrium. When this occurred the absorbance of this reaction was
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Unformatted text preview: measured. The absorbance measurement took the absorbance value of NADH, the product of NAD+ accepting hydrogen. This value used to take the equilibrium concentration of NADH by using the molar absorptivity value for NADPH found by creating a standard curve. The concentration of NADH was found to be 9.1E-5 M, which was also the concentration of acetaldehyde as well as the change in the initial concentration of ethanol and NAD+. From this and the equation for the reaction, the equilibrium constant K was calculated. It turns out that the calculated K value for the experiment performed by my group was about .007. What this shows is that alcohol is converted to acetaldehyde but amount converted is not that great. In the real world, this means that it takes a decent amount of NAD+ and alcohol dehydrogenase to detoxify oneself from a night of drinking. This could in fact be the reason why it takes a long time for people to sober up when very intoxicated....
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This note was uploaded on 11/22/2009 for the course CLAS 170 taught by Professor Staley during the Spring '07 term at Maryland.

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abstract for experiment 3 - measured The absorbance...

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