MIMG101%20Lecture%2020%20HGT%20Overheads

MIMG101%20Lecture%2020%20HGT%20Overheads - Conservative =...

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OUTLINE MIMG101: Horizontal Gene Transfer in Bacteria • Mechanisms of Gene Transfer • Transformation: direct DNA uptake from environment • Conjugation: DNA transfer between two bacteria via cell-cell contact • Transduction: Virally-mediated gene transfer • Discovery of plasmids • Hfr mating • Transposable elements
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Insersion Sequences (IS): General Structure transposase Insertion Sequence (IS) = transposase gene flanked by 2 inverted repeat sequences
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Unformatted text preview: Conservative = cut and paste retain only one copy Replicative = copy and paste Duplicate original Excise/Copy Integrate elsewhere Transposons: General Structure transposase transposase TetR Transposon (Tn) = 1 or more DrugR genes flanked by 2 insertion sequences (IS) Tn DrugR Tn3 AmpR Tn5 KanR Tn10 TetR Transposable element consequences:-Gene mutations-Genome evolution-Spread of DrugR...
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MIMG101%20Lecture%2020%20HGT%20Overheads - Conservative =...

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