LectureSlidesDevelopment_1

LectureSlidesDevelopment_1 - Transmission Electron...

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Unformatted text preview: Transmission Electron Micrographs of Bacillus subtilis Vegetative Cells and Spores spore Vegetative cell Stages of Bacillus subtilis sporulation spore vegetative cell time under starvation conditions Sigma-E is present prior to asymmetric cell division, and then confined to the mother cell after division. after asymmetric septation blue=DNA red=SigE Pogliano et al. 1997 J. Bacteriol. 179:3331-3341 predivisional cell Sigma-F is present in both the mother cell and forespore. overlay of panels B & C red=SigE Pogliano et al. 1997 J. Bacteriol. 179:3331-3341 green=SigF Forespore and Mother Cell Specific Gene Expression PsigF-gfp Webb et al. 1995 J. Bacteriol. 177:5906-5911 PsigE-gfp Control of SigF by an anti-antisigma and a phosphatase Anti-anti-sigma Anti-sigma phosphatase Hilbert DW, Piggot PJ. Microbiol Mol Biol Rev. 2004 Jun;68(2):234-62. Cascade of Sigma Factors Hilbert DW, Piggot PJ. Microbiol Mol Biol Rev. 2004 Jun;68(2):234-62. +nitrate -nitrate Zhang et al. 2006 Mol. Microbiol. 59:367-375 WT (+NO3) multicopy patS (-NO3) Yoon & Golden 1998 Science 282:935-938 WT (-NO3) ∆patS (-NO3) PatS-GFP expression is limited to the heterocyst Golden & Yoon 2003 Curr. Opin. Microbiol. 6:557-563 ...
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