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Slide_Sigmas_2009

Slide_Sigmas_2009 - Transcription Regulation Sigma Factors...

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Transcription Regulation: Sigma Factors Outline 1. Control of transcription initiation in response to a change in the environment. 2. Sigma Factors 3. Anti-sigma factors
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Stress Stress in an abiotic factor or set of factors that limits the production of biomass. Examples of stress factors are unfavorable temperature, pH, nutrient availability, water availability, oxygen concentration, etc.
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Sense and Adapt to Stress E. coli grows optimally at 37°C and dies at 55°C. What happens to E. coli at 42°C – a stress condition? • Is E. coli sensing this non-optimal temperature and responding to limit the damages caused by the high temperature? If so, then E. coli grown at 42° should be better able to deal with the effects of 55° than E. coli grown at 37°.
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How is E. coli able to sense and adapt to the stress of 42°? Ask what changes about cells grown at 37° versus 42° - specifically what proteins change? Cells were treated with 35 S-methionine for 1 min to label the most highly expressed proteins in the cell. These proteins were analyzed by SDS-PAGE. The proteins were visualized by exposing the SDS- polyacrylamide gel to film.
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