lab7 data - Native 0 20 40 60 80 100 120 140 160 180 200...

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Unformatted text preview: Native 0 20 40 60 80 100 120 140 160 180 200 220 240 SLOPE 0.00000% 0.00625% 0.01250% 0.02500% 0.05000% 0.10000% 0 0 0 0 0 0 0 0.02 0.06 0.02 0.02 0.01 0 0.08 0.11 0.08 0.08 0.05 0 0.14 0.18 0.15 0.15 0.11 0 0.2 0.23 0.22 0.2 0.16 0 0.25 0.29 0.28 0.25 0.2 0 0.31 0.35 0.34 0.3 0.24 0 0.36 0.4 0.39 0.35 0.28 0 0.42 0.45 0.45 0.4 0.31 0 0.47 0.51 0.5 0.44 0.35 0 0.53 0.56 0.56 0.49 0.38 0 0.58 0.61 0.61 0.53 0.41 0 0.63 0.66 0.66 0.58 0.45 0 0 0 0 0 0 Denatured 0.00000% 0.00625% 0.01250% 0.02500% 0.05000% 0.10000% 0 0 0 0 0 0 0 20 0 0.01 0.01 0.02 0.03 0.03 40 0 0.06 0.07 0.09 0.12 0.13 60 0 0.12 0.15 0.21 0.24 0.26 80 0 0.2 0.25 0.32 0.37 0.38 100 0 0.28 0.34 0.43 0.49 0.5 120 0 0.36 0.42 0.53 0.6 0.59 140 0 0.44 0.51 0.63 0.7 0.68 160 0 0.51 0.59 0.72 0.8 0.77 180 0 0.58 0.67 0.8 0.89 0.85 200 0 0.65 0.74 0.9 0.99 0.93 220 0 0.71 0.82 0.99 1.08 1.01 240 0 0.78 0.89 1.08 1.16 1.07 SLOPE 0 0 0 0 0.01 0 Velocity vs. H2O2 Concentration 0.01 0.01 0 Velocity (m/s) 0 Row 16 Row 32 0 0 0 Velocity (m/s) 0 Row 16 Row 32 0 0 0 0.00000% 0.02000% 0.04000% 0.06000% 0.08000% 0.10000% 0.12000% H2O2 Concentration (%) 1%=.3M Vmax Km % Km Native 0 0 Denatured 0.01 0.01 0.12 0.24 Enzyme Velocity- 0% 1 0.9 0.8 0.7 0.6 Column D Absorbane 0.5 Linear Regression for C Column D 0.4 0.3 0.2 0.1 0 0 f(x) = 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .00625% 0.9 f(x) = 0x - 0.06 0.8 f(x) = 0x - 0.02 0.7 0.6 Column E 0.5 Absorbane Linear Regression for C olumn E 0.4 Linear Regression for 0.3 0.2 Row 16 Row 32 0.1 0 0 50 100 150 Time (sec) 200 250 300 Absor 0.4 Linear Regression for 0.3 0.2 Row 16 Row 32 0.1 0 0 50 100 150 Time (sec) 200 250 300 0.12000% Enzyme Velocity- .0125% 1 f(x) = 0x - 0.06 0.9 0.8 f(x) = 0x + 0.01 0.7 0.6 Column F Linear Regression for Absorbane 0.5 C olumn F Linear Regression for 0.4 0.3 0.2 0.1 0 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .025% 1.2 f(x) = 0x - 0.05 1 0.8 f(x) = 0x - 0.02 Column G Linear Regression for Absorbane 0.6 Column G Linear Regression for 0.4 0.2 0 0 50 100 150 200 250 300 Linear Regression for Absorbane 0.6 Column G Linear Regression for 0.4 0.2 0 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .05% 1.4 f(x) = 0.01x - 0.04 1.2 1 0.8 Absorbane f(x) = 0x - 0.01 0.6 Column H Linear Regression for Column H Linear Regression for 0.4 0.2 0 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .1% 1.2 f(x) = 0x - 0.02 1 0.8 Column I Linear Regression Absorbane 0.6 f(x) = 0x - 0.01 Column I Linear Regression 0.4 0.2 0.8 Column I Linear Regression Absorbane 0.6 f(x) = 0x - 0.01 Column I Linear Regression 0.4 0.2 0 0 50 100 150 Time (sec) 200 250 300 Enzyme Data - N2 & P5 -G2 Column D Linear Regression for Column D Column D 17-Oct-06 0.01250 0.0004 0.0004 0.0111 0.0007 0.0004 0.0500 0.0007 0.0002 Native Enzyme Group 0.00000 0.00625 0.0003 0.0002 0.0053 0.0006 0.0003 0.0300 0.0002 0.0003 0.02500 0.0007 0.0005 0.0150 0.0006 0.0006 0.1000 0.0009 0.0003 0.05000 0.0007 0.0006 0.0131 0.0006 0.0006 0.1000 0.0010 0.0003 0.10000 0.0007 0.0004 0.0115 0.0008 0.0005 0.0800 0.0009 0.0007 1 2 3 4 5 6 7 8 Mean StDev 0.0000 0.0000 0.0000 0.0003 0.0000 0.0000 0.0000 0.0000 300 0.0000 0.0001 0.0047 0.0104 0.0080 0.0174 0.0148 0.0348 0.0146 0.0348 0.0119 0.0278 Denatured Enzyme - NaCl Group 0.00000 0.00625 0.01250 0.0004 0.0000 0.02500 0.0004 0.0005 0.05000 0.0005 0.0004 0.10000 0.0004 0.0003 2 7 Mean StDev 0.0000 0.0000 0.0014 0.0002 0.0000 0.0000 0.0008 0.0008 0.0002 0.0003 0.0005 0.0001 0.0005 0.0001 0.0004 0.0001 Denatured Enzyme - Boiling water Group 0.00000 0.00625 0.01250 Column E Linear Regression for Column E Column E Linear Regression for Column E 0.02500 0.0000 0.0000 0.05000 0.0000 0.0000 0.10000 0.0000 0.0000 1 8 Mean StDev 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.000005 0.000015 0.000003 0.000010 0.000015 0.000004 0.000006 0.000021 0.000004 0.000014 0.000021 0.000005 Denatured Enzyme - pH 9 Group 0.00000 0.00625 0.01250 0.0019 0.02500 0.0024 0.05000 0.0025 0.10000 0.0022 3 0.0000 0.0011 300 Linear Regression for Column E 4 5 6 300 0.0006 0.0000 0.0000 0.0008 0.0002 0.0200 0.0004 0.0002 0.0200 0.0006 0.0003 0.0300 0.0007 0.0003 0.0300 0.0004 0.0002 0.0300 Mean StDev 0.00015 0.00030 0.00553 0.00966 0.00563 0.00961 0.00833 0.01448 0.00838 0.01445 0.00820 0.01456 Column F Linear Regression for Column F Column F Linear Regression for Column F 300 Column G Linear Regression for Column G Column G Linear Regression for Column G 300 Linear Regression for Column G Column G Linear Regression for Column G 300 Column H Linear Regression for Column H Column H Linear Regression for Column H 300 Column I Linear Regression for Column I Column I Linear Regression for Column I Column I Linear Regression for Column I Column I Linear Regression for Column I 300 Enzyme Velocity vs. [H2O2] - Aggregate 0.02 0.01 0.01 0.01 Native Enzyme 0.01 Denatured Enzyme - NaCl Denatured Enzyme - Boiling water Denatured Enzyme - pH 9 0.01 Enzyme Velocity 0 0 0 0 0.02 0.04 0.06 0.08 0.1 0.12 H2O2 Concentration (%) 1%=.3M Native NaCl Heat pH 9 Vmax Kmax % Kmax 0.0148 0.01 0.4 0.0008 0 0.12 0 0 0 0.01456 0.01 0.15 nzyme d Enzyme - NaCl d Enzyme - Boiling water d Enzyme - pH 9 Enzyme Velocity vs. [H2O2] - Aggregate 0.02 0.01 0.01 0.01 Native Enzyme 0.01 Denatured Enzyme - NaCl Denatured Enzyme - Boiling water Denatured Enzyme - pH 9 0.01 Enzyme Velocity 0 0 0 0 0.02 0.04 0.06 0.08 0.1 0.12 H2O2 Concentration (%) Velocity vs. H2O2 Concentration 0.01 0.01 0 Velocity (m/s) 0 Row 16 Row 32 0 0 Velocity (m/s) 0 Row 16 Row 32 0 0 0 0 .00000% 0.02000% 0.04000% 0.06000% 0.08000% 0.10000% 0.12000% H2O2 Concentration (%) 0% 1 0.9 0.8 0.7 0.6 Column D Absorbane 0.5 Linear Regression for Column D Column D l ng water 0.4 0.3 0.2 0.1 0 0 f(x) = 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .00625% 0.9 f(x) = 0x - 0.06 0.8 f(x) = 0x - 0.02 0.7 0.6 Column E 0.5 Absorbane Linear Regression for Column E Column E 0.4 Linear Regression for Column E 0.3 0.2 Row 16 Row 32 0.1 0 0 50 100 150 Time (sec) 200 250 300 0.2 Row 16 Row 32 0.1 0 0 50 100 150 Time (sec) 200 250 300 0% Enzyme Velocity- .0125% 1 f(x) = 0x - 0.06 0.9 0.8 f(x) = 0x + 0.01 0.7 0.6 Column F Linear Regression for Column F Absorbane 0.5 Column F 0.4 Linear Regression for Column F 0.3 0.2 0.1 0 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .025% 1.2 f(x) = 0x - 0.05 1 0.8 f(x) = 0x - 0.02 Column G Linear Regression for Column G Absorbane 0.6 Column G Linear Regression for Column G 0.4 0.2 0 0 50 100 150 Time (sec) 200 250 300 Absorb Linear Regression for Column G 0.4 0.2 0 0 50 100 150 Time (sec) 200 250 300 Enzyme Velocity- .05% 1.4 f(x) = 0.01x - 0.04 1.2 1 0.8 Absorbane f(x) = 0x - 0.01 0.6 Column H Linear Regression for Column C olumn H Linear Regression for Column 0.4 0.2 0 0 50 100 150 T ime (sec) 200 250 300 Enzyme Velocity- .1% 1.2 f(x) = 0x - 0.02 1 0.8 Column I Linear Regression for Colum Absorbane 0.6 f(x) = 0x - 0.01 Column I Linear Regression for Colum 0.4 0.2 0 0 50 100 150 200 250 300 Linear Regression for Colum Absorbane 0.6 f(x) = 0x - 0.01 Column I Linear Regression for Colum 0.4 0.2 0 0 50 100 150 Time (sec) 200 250 300 Column D Linear Regression for Column D Column D C olumn E Linear Regression for Column E Column E Linear Regression for Column E C olumn F Linear Regression for Column F Column F Linear Regression for Column F Column G Linear Regression for Column G Column G Linear Regression for Column G 0 Linear Regression for Column G 0 Column H Linear Regression for Column H Column H Linear Regression for Column H 0 Column I Linear Regression for Column I Column I Linear Regression for Column I 300 Linear Regression for Column I Column I Linear Regression for Column I 300 1 0.9 0.8 0.7 0.6 Absorbane 0.5 0.4 0.3 0.2 0.1 0 0 f(x) = 0 R² = -1.#NAN 50 100 0.9 f(x) = 0x - 0.06 0.8 R² = 0.99 0.7 0.9 f(x) = 0x - 0.06 0.8 R² = 0.99 0.7 0.6 0.5 Absorbane 0.4 0.3 0.2 0.1 0 0 50 100 1 f(x) = 0x - 0.06 0.9 R² = 1 0.8 0.7 0.6 Absorbane 0.5 0.4 0.3 0.2 0.1 0 0 50 100 1.2 f(x) = 0x + 0.02 R² = 1 1 0.8 Absorbane 0.6 0.4 0.2 0 0 50 100 1.4 f(x) = 0.01x - 0.04 R² = 1 1.2 1 0.8 Absorbane 0.6 0.4 0.2 0 0 50 100 0.2 0 0 50 100 1.2 f(x) = 0x - 0.02 R² = 0.99 1 0.8 Absorbane 0.6 0.4 0.2 0 0 50 100 0% Denatured Enzyme Column D Linear Regression for Column D 50 100 150 Time (sec) 200 250 300 .00625% Denatured Enzyme x - 0.06 .99 .00625% Denatured Enzyme x - 0.06 .99 Column E Linear Regression for Column E 50 100 150 Time (sec) 200 250 300 .0125% Denatured Enzyme - 0.06 Column F Linear Regression for Column F 50 100 150 Time (sec) 200 250 300 .025% Denatured Enzyme Column G Linear Regression for Column G 50 100 Time (sec) 150 200 250 .05% Denatured Enzyme x - 0.04 Column H Linear Regression for Column H 50 100 150 Time (sec) 200 250 300 50 100 150 Time (sec) 200 250 300 .1% Denatured Enzyme 2 Column I Linear Regression for Column I 50 100 150 Time (sec) 200 250 300 ...
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This note was uploaded on 11/24/2009 for the course ENGINEERIN 4283 taught by Professor Smith during the Spring '09 term at American InterContinental University Dunwoody.

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