3444Lab - Fall 2009 3444 (Sections 003- 009) General...

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1 Fall 2009 3444 (Sections 003- 009) General Microbiology Lab Syllabus Graduate TA: Email: Office Hours/Location: Room: Class day & Time: Monday – Thursday 2:00 – 4:50 p.m. Lab Manual: Microbiology Laboratory Theory and Application: Second Edition. Morton Publishing Company Supplements: UTA 3444 Microbiology Lab Handouts Available for purchase on the first day of lab from the GTA. Price: $10.00 Lab Kits for Microbiology Lab – Purchase Price: $10 available for Purchase the first day of lab from GTA. Lab # Dates Topic/Title Reading 1. Aug. 31 – Sept. 4 Safety and Laboratory Guidelines . .................................................................. p.1-5 Media Prep . ..................................................................................................... .Ex. 1-1 Evaluation of Media………………………………………………………………….Ex. 2-5 Microscopy . ..................................................................................................... .Ex. 3-1 Wet Mount Preparation……………………………………………………………. ..p. 76 Ubiquity of Microorganisms…………………………………………………………Ex. 2-1 Bacteria Structure … . ...................................................................................... p. 77-80 Aseptic Techniques…………………………………………………. .……………. .Ex. 1-2 2. Sept. 14 - 18 Observation of Environmental Isolation Plates Colony Morphology ………………………………………………………………. .Ex. 2-2 Growth Patterns on Slants………………………………………………………. ..Ex. 2-3 Growth Patterns in Broth…………………………………………………………. .Ex. 2-4 Staining I Smear Preparation. . ..................................................................................... Ex. 3-4 Simple Staining . ........................................................................................... Ex. 3-4 Gram Staining . ............................................................................................. Ex. 3-6 Acid-Fast Staining: Ziehl-Neelsen Method . ................................................. Ex. 3-7 3. Sept. 21 - 25 Gram Stain and Microscope Practical Staining II & Cultivation Capsule Staining . ......................................................................................... Ex. 3-8 Endospore Staining the Schaeffer-Fulton Method . ...................................... Ex. 3-9 Pure Culture Techniques Streak Plate Methods of Isolation ............................................................... Ex. 1-3 Quadrant Streak Method …………………………………………………. . p. 20-21 Examples of streaks on page 36-37 T-Streak…………………………………………………………………. . . ... Handout
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This note was uploaded on 11/24/2009 for the course BIOL 3444 taught by Professor Frye during the Spring '08 term at UT Arlington.

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3444Lab - Fall 2009 3444 (Sections 003- 009) General...

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