100L-F09_WEEK%206_Metabolism

100L-F09_WEEK%206_Metabolism - What is Metabolism? What is...

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hat is Metabolism? What is Metabolism? he Greek etabole meaning change The Greek metabole , meaning change • It is the totality of an organism's chemical processes to maintain life. - Catabolism - Anabolism
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arbon Source Carbon Source - The most Carbon source of bacteria is “Glucose” - The major carbohydrate-metabolizing pathway are 1. Embden–Meyerhof–Parnas (EMP) pathway, glycolysis 2. Entner–Doudoroff (ED) pathway Pentose phosphate (PP) pathway 3. Pentose phosphate (PP) pathway 4. Tricarboxylic acid cycle, TCA cycle, Kreb’s cycle, citric acid cycle
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Carbohydrate Metabolism 1. Embden–Meyerhof–Parnas (EMP) pathway, glycolysis
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Carbohydrate Metabolism 2. Entner–Doudoroff (ED) pathway
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Carbohydrate Metabolism 3. Pentose phosphate (PP) pathway
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able 1: istribution of mbden eyerhof arnas MP) ntner Table 1: Distribution of Embden–Meyerhof–Parnas (EMP), Entner– Doudoroff (ED), and pentose phosphate (PP) pathway in bacteria rganism MP D P Organism EMP ED PP Pseudomonas aeruginosa - +i - Enterococcus faecalis + +i + almonella phimurium Salmonella typhimurium + +i + Bacillus subtilis + - - Escherichia coli + +i + Yersinia pseudotuberculosis + +i - Remark: + = Present; – = not present. i = inducible
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Carbohydrate Metabolism Formation of intermediates of the Embden– Meyerhof–Parnas (EMP) and Entner–Doudoroff (ED) pathway from carbohydrates other than glucose
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Carbohydrate Metabolism 4. TCA cycle
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BIOENERGETICS - Review NRG – ability to do work (units of kJ) Free NRG (G) – NRG available to do useful work (potential NRG; not lost as heat) actants roducts NRG `=G Free NRG reactants products NRG G o = G o f P – G o f R “products minus reactants” G o f = Free NRG of formation G o ` > 0 G o ` < 0 (+ G o `) (- G o `) endergonic exergonic OW? Need NRG to proceed Release NRG (cell may SAVE) HOW? REDOX RXNS (exchange of e¯)
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NRG Conservation ~ REDOX RXNS Oxidation – loss of e¯ from DONOR eduction ddition of e¯ to ACCEPTOR Oxidation Reduction – addition of e to ACCEPTOR Reduction DONOR (X red ) (Y red ) (X ox ) ACCEPTOR (Y ox ) “Electron flow”
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Reduction Potential (E 0 `) – tendency for substance to become reduced (gain e¯) – measured in Volts (V) – expressed as reduction rxns for “half-rxns” ~ REDOX COUPLE (X ox / X red ) reduction Good donors E 0 ` (V) - NRG consuming CO 2 /glucose (-0.43) AD+/NADH (- .32) X ox / X red ( ) oxi d rxns NAD+/NADH ( 0.32) “fall” 0 ation rx n eduction r +1.14 +1.25 E 0 ` = ½ O 2 /H 2 O (+0.82) Y ox / Y red s re Good acceptors ( + ) NRG yielding
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e¯ carriers I. Free diffusible coenzymes/non-protein cofactor EX: NAD+, NADP+ ttached to CM (prosthetic groups on proteins) II.
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This note was uploaded on 11/25/2009 for the course MIMG 100L taught by Professor L during the Fall '09 term at UCLA.

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100L-F09_WEEK%206_Metabolism - What is Metabolism? What is...

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