Lecture21-2009

Lecture21-2009 - EXAM 2 MONDAY OCTOBER 26 8:30-10 PM Javits...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
10/20/09 1 EXAM 2: MONDAY, OCTOBER 26, 8:30-10 PM • Javits 100 • Covers lectures 10-19 • Approximately 10 points/lecture: – 80 points from Dr. Simon’s lectures. – 20 points from Dr. Haltiwanger’s lectures. • In class review session: Friday, Oct. 23. • TA review: Wednesday, Oct. 21, 6:50-8:00 pm, Javits 100. Glycolysis II Lecture 21 Chapter 15, Sections 3 and 5 Figure 15-1 Preparatory Stage Payoff Stage Page 497 Reaction 6: GAP dehydrogenase Dehydrogenases: involved in oxididation/reduction reactions. Couples favorable oxidation to unfavorable phosphorylation through covalent intermediate: thioester
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
10/20/09 2 Figure 15-9 GAP dehydrogenase mechanism Figure 15-9 part 1 δ - δ + Charge separation Figure 15-9 part 2 δ - δ + Charge separation Easier to extract H - from thiohemiacetal than carbohyl due to dissipation of charge separation. Figure 15-9 part 3 Energetically favorable oxidation Energy from oxidation conserved in thioester
Background image of page 2
10/20/09 3 Figure 15-9 part 4 Phosphorolysis: Conserves energy of thioester (originally from oxidation) in acyl-phosphate Figure 15-9 part 5 (Acyl-phosphate) Figure 15-9 GAP dehydrogenase mechanism Figure 15-8 Experiments supporting GAPDH mechanism (inactive) GAPDH catalyzes Pi exchange
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
10/20/09 4 Page 499 Reaction 7: Phosphoglycerate kinase Substrate level phosphorylation PGK catalyzes a coupled reaction Favorable hydrolysis of 1,3-BPG coupled to unfavorable phosphorylation of ADP 1,3-BPG + H 2 O -----> 3PG + Pi Δ G o ’ = -49.3 kJ/mol ADP + Pi -----> ATP + H 2 O Δ G o ’ = +30.5 kJ/mol _________________________________________________ 1,3-BPG + ADP -----> 3PG + ATP Δ G o ’ = -18.8 kJ/mol GAP + Pi + NAD + -----> 1,3-BPG + NADH + H + Δ G o ’ = +6.7 kJ/mol 1,3-BPG + ADP -----> 3PG + ATP
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/26/2009 for the course BIO 361 taught by Professor Lake during the Fall '08 term at SUNY Stony Brook.

Page1 / 12

Lecture21-2009 - EXAM 2 MONDAY OCTOBER 26 8:30-10 PM Javits...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online