Trimyristin Protocol

Trimyristin Protocol - Experiment 3 TRIMYRISTIN FROM NUTMEG...

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Experiment 3 TRIMYRISTIN FROM NUTMEG Trimyristin In this experiment, you will isolate a single compound, trimyristin, from among the complicated mixture of natural products in its source, nutmeg. The procedure is designed to separate trimyristin from contaminants and improve its purity. As you work on this experiment, think how each step contributes to an overall goal. Here is a brief summary of the Trimyristin experiment: 1) The ground nutmeg is treated with the solvent ether. The solution containing trimyristin is filtered so as to remove the insoluble plant material. The solvent is evaporated, leaving a residue of crude trimyristin. 2) The crude trimyristin is dissolved in the hot solvent acetone. When the solution is cooled, the trimyristin crystallizes out, leaving in solution the majority of the (colored) impurities. Background Reading Review the theory, the general procedure, and the related technique (rotary evaporation) of extraction in sections 9.2 and 9.5. Review the technique for small-scale crystallization in section 7.4. Properties of New Materials Name Appearance Properties Comments Trimyristin Colorless solid Melting point 56 o C -------- Diethyl ether (ether) Colorless liquid Boiling point 35 o C Highly flammable and mildly toxic Acetone Colorless liquid Boiling point 56 o C Highly flammable and mildly toxic CH 3 (CH 2 ) 12 COOCH 2 CH 3 (CH 2 ) 12 COOCH CH 3 (CH 2 ) 12 COOCH 2
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Experimental Procedure Part A Extraction Step in procedure Comments 1) Weigh out 3.0 g ground nutmeg. Watch significant figures. 2) Transfer the nutmeg to a 50 mL Erlenmeyer flask. 3) Measure 25 mL ether by pouring into a graduated cylinder. The term “ether” can be confusing because there is more than one kind. When used alone, the term means “diethyl ether.” The pipet provided on the solvent bottle is for a transfer of a small or exact amount and need not be used here. 4) Add the ether to the nutmeg. Swirl the mixture often over a period of about l5 minutes while you set up for the filtration in step 6. This step extracts most of the trimyristin from the nutmeg into the ether. You should gently cover the flask with a cork in between swirling to minimize evaporation. 5) Pre-weigh a 100 mL rbf and clamp it to a ring stand. Set into the rbf a stemless funnel with a fluted fast-flow filter paper. Fast-flow filter paper will be distributed by the TA.
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Trimyristin Protocol - Experiment 3 TRIMYRISTIN FROM NUTMEG...

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