Exam1Fall09key - mxmB mmmc=m 2.3:” umxmo 33mm £35:5 3:5...

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Unformatted text preview: mxmB \_ mmmc=m 2.3:” umxmo 33mm? £35 :5 3:5 15:86” 3 r92 mooaufi . > 3:99 :3 m. mco<m z u 3 m 33%... mo-.. ow z n he 0 3.69 mem z n mm. D 3st 3:3 2 n A» m Baum” 3 m. 3.92 z u 3 203” <0: :m<m m SE mooqm or: 2 in? .2: "Em 883 W 55653 Ho am 55:: on? we .0055. mos mooqmm m2... 52.25: 0: :5 .3” 3mm 0* <9... 883.. Osmox =5 manEo: man mam 3m 2" :53 .m m: 02.9.. x3 <3: cm woman. 0: :5 563.3 3 200:. Name: K3 2:128 Fonda ental Genetics Exam 1 Sept. 24, 2009 Write or diagram your answers in the space provided. For genetics problems, show all your work. You may get partial credit for an incorrect answer if you do. The approximate amount of time to spend on each question is provided in the brackets. The exam is worth 99 Qts. 1. [6minl Graigch by Sarah D, a. A diploid organism has 8 chromosomes (2n = 8). In the table below, fill—in the # of DNA molecules in one body cell at the different stages of the mitotic cell cycle or in one germline cell at the various stages of meiosis. 0‘ 5 P‘, flaws w W # of DNA Molecules at (31 ' re—S C32 ost~«S Posthitosis After Meiosisl After MeiosislI Body CelliMitosis <3 b 3 not agplicable not applicable ‘3 8 4 b. The dividing cell shown below is from a diploid organism where 2n n 4. Is this cell in metaphase of mitosis, metaphase 101C meiosis, or metaphase ii of meiosis? In one sentence provide your reasoning. Germline CellfMeiosis not applicable Meta Pine-Se oil: M l tools “The, cell “‘15 (fl/WrameSOl/vlafi Meiosis: ~ wows have it .—~ “ H on Ell/W'MOS ' as: 2. [9 min] Consider the diploi cell below (211 m 4). The cell is a meiocyte 31% Walll ‘ 0' OW" SOOU. 111132116 {11610518 . a. Diagram the chromosomes and genotypes (alleles) of all possible gametes if there was no cross—over. \ \ . I . . / l Pi", each b. List all possible genotypes of each of the gametes if there was a single crossover between gene “D” and the centromere. ADE Abe stale cull?— AAE Ase QUE aBe c. Assuming that there is _n_g crossingover, what would be the probability of parents that both have the above genotype (AaDdEe) producing an offspring with genotype AA DD ee‘? (Aligned lvtttléfildmllfl ofr' theeell eqyfifior‘ [59+ l Pi. pairs 01?- clrtvomsomee aligned] Abe gamete $ch 0’? x Abe gamete 9rer 2 1 1/4 X Z 3 1/319 i G r as eel a, Sarah o. f 3. [14 min} Bevo, a prize longhorn bull, is pure-breeding for reddish-orange coat color and long horns. “if; He is mated with a series of prize females aEi of which are pureubreeding for White coat color and short horns, Much to the surprise of the Longhorn fans, all of the offspring of these mating are short—horned and have an intermediate coat coior cailed “roan”. f; 3. Using C and c to designate the alleles for coat color and H and h to designate the alleles for horn ‘2 length, what are the genotypes of Bevo and the females to which he was mated? Bevo: l Pl," (7“ Females: 00 l What is the most likely explanation for the coat color observed in the F1 offspring? InCQMPlfltCi: oominavxce Q pts. r? , " c. if the F1 males and females are mated, what phenotypes (and ratios thereot) are expected in the F2 offspring? You can assume that coat color and horn length are independently asserting traits. Collin x Catlin .z F" {3% Hw 9,10 Draafiejshorl: / horfl ‘I/ . 4 CC \ ‘4 Mi We arrange) lfm 0‘?- 5/ ‘3‘.” 3 i r 1/; CC i i 4 /<5 room, 6&3? Z» We Vs seen, We W Z; (LC/5’4 H“ 3/)b white} short “fix. mm 1/4 w" Vito whiteglena M n i l per correct pheneiyeic close “3’ its ratio) g d. If the F1 females are mated back to Bevo (a scenario not unheard of in animal breeding), what i offspring and proportions thereof are expected? Clo x (1C rm )4 orange, short hero )4 u long hem ' 2% roar», Short ham l“ ya “ long ham 4 Pl? 2 Graded b3 Di". Leéclfi‘t 4. [12 min} The pedigree below is for inheritance of a skeletal disorder. This condition is rare in the general population. 22 I O 1 2 II I I I I O I 1 2 3 4 5 6 ’F 8 9 HI I O O O I O I O 1 2 3 4 5 6 ‘? 8 9 10 11 12 13 14 15 W O I O I ' - I I . . i 2 3 4 5 6 '3’ 8 9 it} a. What is the most likely mode of inheritance of this trait: autosomal dominant, autosomal recessive, . — n ed dominant, r X—linked recessive? Provide two explanations in support of your answer. (api‘é- The t’cv‘m‘i: 1'5 seen m every emerge-hem} eve-etichjh waive, axed wot beiwa brought M by pfiefo 5; who marry {vile "rifle $omily. This indicates dommamei “The Rex; Jre Marked dam. is that a“ QWeeted MCQES P053 0"" SUM had: 3:0 Gmid and ALL daughters . Theee daugkfif-V’S Fags 0“ We emit ’{o New; :23? their song am “50070 e? their“ daughterg b. How can other modes be excluded? Mmfit have Auloegmal down. excluded by) stair-shed whikeimess a:th men with {walk meet“ pass H: is a saw. We expeelc them 10 pass s’c £0 children 5 oi? belch ewes i? auioficmal (i-OV‘mmiv‘Ci. Makeseml rec-egg ceiild week i"? earners maimed; M11} gawkng bur were told {he limit is; rare in time gememl pa antennae luck 6? flopping fieeemmme also makes aw‘rosa reefs—s, “nuke . Mini-(ed recess. 15 de fiwfivel Exflmifd "It C'cmmi be Wed? 510%. Fe melee ID; at» 3&8 would have i J lee Xdficd mod have. all (9M early) aroma {2. Given your response {0 4a, what are the genotypes of the f0 owing individuals? ‘SGflS. I-E: yaw 1-2: mm 3 11-8: III—14: IV—S: 30) 1d IV-9: Kay 9: skeletal disorder (is harm! allele 3 Graded by Dr; Leiclxt 5. [14 min]. in mot s, a cross (Cross 1) of a light colored female from a pure breeding line to a dark colored male from a pure breeding line produces alE dark F1 offspring. The reciprocal cross (Cross 2) of a dark colored female to a light colored male produces light colored F 1 females and dark colored Fl males. a, Explain these results by diagramming both. Cross 1 and Cross 2. Be sure to indicate which genotypes are females and which are males. Ci‘OSf-E 1? 9K “EMMA? M Z Cmsgtg; W61? 2: [109100027 . r 5 T6. um; mg mg?” g X W‘lwéfi’)’ P 3 dark a: he per cross \L 9 (law. is ' LODde dark mower 07’ dark dominafll: DZ 0 a 0 "V 'tollghl: w T Clark W Z + Mal/ll: coler 1“ “0W5; Er) are Refemaamezlito} 0W6 V‘O'Wfiama’l'llfin N01” E: For an pellets, gm need la show Q Met: «1 (£1?th Salt (EMFWKS; as 0M3 owe Carries ame‘rbr bod. Color (D: Wk, (blight ) b. What offspring, and proportions thereofi are expec ed if Eight Fl femaEes from Cross are allowed to mate with their dark male sibs? WK? x mm 0” l; DOD M06 OW Clark V7. 05? males dark, i/:2 05? Males light 4 PK WW4 07' light 7; ‘* ‘Femlcs “ , lg " Rimles “ L0“ 2 5? dark . ml Z 9r . ighl: .. c. What offspring, and proportion“ eof, are expected ll’ dark F 1 males from Cross 2 are mated to the dark females from the pure breeding dark line? WW 0’7 x fix?— 4' pie: \1/ mm 0” I WW; 0’" an dark 5? l ratio 0? dark: If N; overall 3 W52 ll; dark LC)th 7 All d7 clerk g; M“ 4 (/15; light, 1(2.? (lack (avoided by Mark H. 6. {12 min} In both humans and Drosophila, XX individuals are females and KY individuals are males. However, XXY individuals of humans are males while for Drosophila XXY individuals are females. 21. Explain the genetic basis for the difference in sexual development of XXY humans and XXY Drosopl'i-ila. Be thorough in your explanation. Huw‘ifii’iéz Mair: developmewi‘ i“ecpi,i{ee.s c; 88946 mike Y C—l’ii‘Q Wflgg‘fgfifi: Coiled SRY. ' 3 P16. 1w. disses/ice 0? SEW, 9? develppmeat GCCVJ‘S lax; deem )RY Wk?ij fiei’w’s 997*" PWCWC hm" C"? Ca Miami/res and eecepicms , mime 3 than ma (lg 133, Qcmvfimx GI; . Cf? sewer-ares. iv’liifirml/exiceeiial Broaepmla: xr‘A micro oeiei‘mmes Sexual Phfim‘iv PC» Y W€€f§€d' Gil-{V fire 07‘ $erhlity. Xzazi 4; a? I; anfiesera—ecfi (defialb 313%. :m {individuch with we: 3 l8 'EuW/‘xad 0%. Which “‘Cuflxg cape? ea 2' 0o : b. In humans, XYY males occur with an incidence of ~1. in 1,000 births. Diagram the meiotic events and fertilization that lead to an XYY embryo. Your diagram should include gamete formation in both parents and should be clearly labeled. Diagram only the sex chromosomes. 39 a?“ 3 (a are-me cradled Ski CW 93* e“ peer-H c. (afiflfix 5 555’: Meicsie ii: J k F ® géfiw‘flfli’iei’i ; 0$X§Y Meicsiel’i Y/ \ \/ \a mile y x y\0iméi{§juv\€iii7fl . six?" iii 0 Q) '1 in ® ® i 0m\/ a {7k 1‘ Miliéi'fifigfl X X 5 . Graded in; Math Hoiioraflk H n 7. {7 min.] In a hypotheticai diploid plant, the g and “h” genes are linked, and are 6 map units apart. Suppose that the FE offspringgf a cross between Gth and ggHH parents are self-poliinated. a. What is the expected frequency of each of the foilowing types of gametes from the F1 heterozygous parents? Oh: .47 [on 47% Q ts. gH’ ° H7 [Ll/7%) P GH: . (/13 g .3 e «03 (3% b. Among the F2 offspring, what proportion is expected to be of the genotype GGHH‘? grab. G Pt e33 >4 Qr‘eO-v. G it gape mm l 4. Pi it: 0:055 “x Oat???) '«1 , 000‘? c. Among the F2 offspring, what proportion is expected to have the Gth genotype? e occurs {:0 get Gcfiilh (Gm 833 x git SpernnDtlgii egg x GM-epet‘cw> fr KER egg x 3h 5P'E’W‘Vt3 a" (an 993 x at: S-Fffm) (Arise a7) ~‘r [At-“H.475 t [Cass-tee.) a [:03 toss): ‘ steep, 8. [7 min.] in Drosophila, the genes jv, se and app have been mapped to the third chromosome and are ordered as shown below. jv se app 7mo- ll mu 3. Suppose that a 3—factor cross involving these three genes is performed. Based on the indicated map distances, what is the expected frequency (rdmhles) of the double crossover class (one crossover between jv and se and a second crossover between 56 and app)? Expect/ed DG-{P‘ Q‘ecg- : (.9736 5i):~ 9077 Q Pisa 13. Suppose in the actual cross, 1012000 double crossover offspring were obtained. Calculate the amount of Interference (1), showing all your work. Exp. in one Mtg-errth C aeees .8577 tr [5:4 (“#55 M) CV95. it Be? :1 it? )KCee-W. o? Ceimide-neezw QiES'DC'VEXg DOE} 1‘“ iD/lb‘ 3- @7197 a Ski/5 he" “3/15; 4“) I»; 1w C.G§.C1:L_.l0k77 3&333 it 4%,, a A? . fl _ , 0r H063 ECU/EX? DCC} :. egg??? 3. 0. tag” Gmtiecl by gater 6'. 9. {24 min}. Two haploid yeast strains are crossed to give the diploid ACD/acd which is then sporulated. Asci are isolated, and tetrads are classified as PD, NPD, or T. You find the following tetrads (number of each type of tetrad indicated in brackets). l 1} EH EV V VE VII ACD aCD ACd aCd acD ACD aCD ACD 3CD AC3) aCD acD ACE) ACD W, acd Acd acD AcD ACd aCd acd 5. b : acd Acd acd Acd ACd aCd Acd [76] [76] [E8] [18] [2] [2] [8] Pl) PD T "V NPD NPD VD a. Label each class of tetracl to indicate whether, with respect to C and D, it is a PD, NPD, or T. (Write beneath the bracket with the number of that type.) b. Based on these results, are C and D on the same (homologous) or on separate chromosomes? Explain. {f they are on the same chromosome, calculate the map distance between them. a?“ Ci» 0 are mm (same oWomcsoMc) PD >vN‘PD m vs. Ll [bf-3 CeD disbm’xee 2: 50 C “F Jr (aNP'D) Mwmwwm 3:.- 50 Jr 34 r: Mil/x. Total @425 c. Here are the same data that were shown above. Label each class of tetrad to indicate whether, with respect to A and C, it is a PD, NPD, or T. t .11 11:. iv V VI. VII , I ACD aCD ACd aCd acD AcD aCD 5.5 pi“? Ace aCD ACD aCD acD AcD ACD acd Acd acD AcD ACd. aCd acd acd Acd acd Acd AC6. aCd Acd {76} ' E76] [18] [18] [2} [2] [8] Pl) NPD Pl) MW) PD NPD "l- d. Based on these reselts, are A and C on the same (homologous) or on separate chromosomes? Explain. If they are on the same chromosome, calculate the map distance between them. A camel are mole. lihkecl. Pp: NPD 3?“ City vs. fit, A me? da'stawce cannot; be: Calculated. e. How do tetrads of eiass HI and IV arise? Separately diagram the events of meiosis that would lead zgfijw—«mrfi both of “1essiefeiiméa7,,,___‘_1_7_ _. ..... .. .. . . .. am (7 A i C d, I 1 "MM; {3‘ If /« h C c: \ \ Me 1 TI: Kuwaiti?” _,, ., ._.._Erw..~v§f\\‘ m 5/ r w 3 w W , g i 3 2 ma 5 t “W? 2 ma Mimi-3r :5 > wit We“ i. A K M -—- M ,__M_."__Afi_~.,.A—»—-———--- ‘ \i_ a W} \\ a C 61 I W / C rosscflfif i“ — WNW—4M- #, M M befifififin 0%) M CR? 5011‘” _ “GR ii- ‘ Meiesfiflt a C: a Somme, as txpr E, w“? »»»»»»»» buck m HA A + a WHW’WWJMW , fix WWW» % homclgqs or? {3 \J‘ C. .z Pcififi gram ,1}: . K M f. How do te-trads of class VII arise? Diagram the events of meiosis that produce this tetrad type. ..., MMMM am“ fir; C D- is: p A C / ‘9‘ C” “‘ QWA we”) \ / A Cwssoves" b€ mifiefl 159/ “i 8% Oeu’c’mm ere, Graded in/ Dr. Latch-i: 16. [15 min] Mike the mixed up microbiologist wants to do a mating experiment with severe} E. coli strains, but he forgot to Iabei his cuiture tubes and now doesn’t know which is which. One of the strains is Hfr, str~5 pr0+ £hr-i-. A second is F+ Str~s pr0+ thr+ and a third is F— str-r pro- thr-. He decides to arbitrarily call the unlabeled strains A, B and C, and mix the strains in all pairwise combinations to see which mixtures wilt give sir-r pr0+ thr+ recombinants as evidence of mating. Suppose that what he labels A is Hfr, sir-s pro-l» [121%, what he labeis B is 17+ sir—s pro—t [hf-F, and what he labels C is F— sir-r pro- thr-. a. Using the table below, record the expected outcomes for the different pairwise mixtures. Can produce str-rpro+ thr+ recombinant offspring (Yes or No) Conjugation will occur Mixture (Yes or No) N Gums but,“ teas ’ Sm§$l€C rent . d. What kind of medium should Mike use to identify (and seiect for) the recombinant bacteria? Be specific. ‘ _ Me di am Hi?! tine Mi stwgte Wt C W» Recail th t Mike oesn’t really know which strain is which (he just arbitrarily called them A, B and C). Describe one way that he couid determine that A is the Hfr strain and B is the F+ strain. You should describe how Hfr and F + strains differ as part of your answer. lite strains tectnSQe-r bacterial gene-s at a high irerfiueeeébeeausc all Cells of d We strain have «the if phasmd migrated 53/1156? Piroiitfsé time/i 'bhttitimme? but which CSVKi:C\BI1/35 at" bacterial ei’xmmggpmai Ft fitted as hate-3995*“ hacierlai Res-ewes Cd: aim-tick indie/t 4:3"? » because in a Mix-beg populei'ieh Wei de met hauethe F plasgue iwtegrateéfiwte We beeteridl chem, and onlfl breeder shame m . ‘Fewcths he News _ _ and Com Wen 'b‘t‘fl'f‘xgg'fii” been. genes, agree; thogieret we commas tells his whlfii {3‘ whim» . . . a ' Ase-mi enemies between he s P“ Wales is that F" Will Mme”?! Wig Fifi-“3m 3: r mt?“ shake we mkefi it. W (e (fleets-rites. Hit stems seldwttraefi‘rer tail??? F Piafim‘éi 53 Nike has a fourth E. coli strain t at arose from his Hfr, Sir-s pro+ thr+ strain that behaves differently P“ waged; than expected in matings. This strain orin transfers thr+ to recipient ceils, never the pr0+ marker. gfgfl¥g k What could explain this, assuming that the pr0+ gene itself has not changed? “We, 5%” 5356mm) which foam/Q, Rem trier Petr" Carpet-hie. {>4}? i:"rtxi’\§-$efiy\j holds pro‘i CRRCEX Wt? is F' the"? The i: piesmid ftKC-rfifidv ire-M arise; \saclretial chem-sesame m ahutmorccise tea- sed {we grow? the twigs/M» The t“ ichd onward coe “bravrfisrer to a i?“ can Camera/ma it 5C1“) Filed. Pre‘i is set the the F’ plasmid. ...
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This note was uploaded on 11/29/2009 for the course BIOLOGY 2:128 taught by Professor Leicht during the Fall '09 term at University of Iowa.

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Exam1Fall09key - mxmB mmmc=m 2.3:” umxmo 33mm £35:5 3:5...

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