ExamII_Fall2007_key - Name:_ CH339K EXAM II Fall 2007 UT...

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1 Name:____________________________________ UT EID: ______________________ CH339K EXAM II Fall 2007 Prof. Marcotte ** SHOW WORK FOR COMPUTATIONAL PROBLEMS OR NO CREDIT!! ** Useful constants: R = 0.0821 L · atm/mol · K N = 6.022 x 10 23 /mol (1) You have isolated a peptide with the following sequence: Ser-Met-Glu-His-Phe-Arg-Trp-Lys-Pro-Val 10pt (a) Sketch the titration curve you would expect for this peptide. Label all pH values 5pt (b) On the isoelectric focusing gel shown below, estimate where you would expect this peptide to migrate (draw the band in on the gel): The pI is ~9.8 = (10.5 + 9.15)/2
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2 10pt (2) Amino acids and peptides A B C D E ___________ __________ __________ __________ __________ Tyr-Lys-Met Gly-Pro-Arg Asp-Trp-Tyr Asp-His-Glu Leu-Val-Phe Which one of the above tripeptides: ____ (a) is most negatively charged at pH 7? ____ (b) will yield DNP-tyrosine when reacted with l-fluoro-2,4-dinitrobenzene and hydrolyzed in acid? ____ (c) contains the largest number of nonpolar R groups? ____ (d) contains sulfur? ____ (e) will have the greatest light absorbance at 280 nm? (3) In a rare Peruvian herb, you’ve discovered a bioactive peptide that stimulates cell growth. In order to characterize it, you first purified the peptide. Shown below is the purification table. 10pt (a) Complete the table by calculating the specific activity, fold-purification, and % yield for each step. Step Total protein Total activity Specific activity Purification Yield (mg) (units) (units/mg) (fold) (%) Homogenate 4650 201 0.43 1 100 Ammonium sulfate 2240 195 0.87 2 97 Heparin- agarose 116 107 0.922 21 53 Orange A
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This note was uploaded on 11/30/2009 for the course CH 55655 taught by Professor Marcotte during the Spring '09 term at University of Texas at Austin.

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ExamII_Fall2007_key - Name:_ CH339K EXAM II Fall 2007 UT...

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