319.homework.september

319.homework.septemb - separately in the presence of each of the intermediates The chart below outlines whether the yeast were able to grow or

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Bio 319 homework questions, August 31 - September 23 Homework questions for Bio 319 Lecture #1 (answers are given after the questions) 1. You have isolated 6 organisms that show a mutant phenotype. Complementation analysis of these organisms gives you the data below. "+" stands for wildtype phenotype. "-" designates the mutant phenotype. 1 2 3 4 5 6 1 - + - + + + 2 + - + + + - 3 - + - + + + 4 + + + - + + 5 + + + + - + 6 + - + + + - How many complementation groups are there? From this analysis, can you figure out the minimum &/or maximum number of genes involved in this phenotype? If so, give the number. If not, why not? Answer: That means that there are a minimum of 4 genes involved in this phenotype. We can't determine the maximum number of genes in the phenotype because we can never be sure that we have isolated a mutant in every gene involved.
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2. A, B, C, D, E, and F are all intermediates along the biosynthetic pathway that leads to substance G. Seven individual yeast mutants in this pathway were isolated and cultured
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Unformatted text preview: separately in the presence of each of the intermediates. The chart below outlines whether the yeast were able to grow (+) or could not grow (-) in the presence of the various intermediates. (Notice that they are all able to grow in the presence of G) Added to the growth media Mutant A B C D E F G 1 + + + + +-+ 2------+ 3-+ +-+-+ 4-+--+-+ 5 + + +-+-+ 6 + + + + + + + 7----+-+ Fill in the following diagram with the intermediates and the genes that produce them in order. -->-->-->-->-->-->G (An incorrect example:) A--> B--> C--> D--> E--> F-->G 1 2 3 4 5 6 Correct Answer:--> F--> D--> A--> C--> B--> E--> G 6 1 5 3 4 7 2 Other homework - answers are in the back of your book Chapter 2: c12, c16, c18, c24, c28, c34 Chapter 3: c24, c30 Chapter 4: c4, c10, c16, c24 Chapter 5: c6, c10, c14, e4, e12, e20 Chapter 7: c6,c16, c18, e4 Chapter 8: c12, c18, c32, e12 Chapter 9: c6, c12, c14, c20, c24, e4 Chapter 10: c14 , e2...
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319.homework.septemb - separately in the presence of each of the intermediates The chart below outlines whether the yeast were able to grow or

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