lectures 10-2 and 10-5

lectures 10-2 and 10-5 - Uses of PCR Amplification of large...

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Uses of PCR Amplification of large amounts of a specific or random DNA from a small sample for various purposes, including: Cloning Making Probes Gene Mapping Mutagenesis Diagnostics/Forensics
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The Replicon Model Replication is controlled by a DNA sequence called a replicator that binds an “Initiator” protein. The initiator protein regulates replication of the DNA DNA synthesis actually begins at the origin, which is usually within or adjacent to the replicator.
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The Roles of the Initiator Initiator binds DNA. Initiator unwinds (a little) DNA at the origin. Initiator recruits other replication proteins.
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Genetic identification of replicators
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The Eukaryotic Cell Cycle The major events of cell division, DNA replication and chromosome segregation, are separated in time, into cell cycle phases. During the Gap phases (G1 and G2) checkpoints ensure that all is well with the DNA and segregation machinery, before allowing DNA replication (S phase) and
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This note was uploaded on 12/01/2009 for the course BISC 13019 taught by Professor Aparicio during the Spring '09 term at USC.

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lectures 10-2 and 10-5 - Uses of PCR Amplification of large...

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