lectures 10-2 and 10-5

lectures 10-2 and 10-5 - Uses of PCR Amplification of large...

Info icon This preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Uses of PCR Amplification of large amounts of a specific or random DNA from a small sample for various purposes, including: Cloning Making Probes Gene Mapping Mutagenesis Diagnostics/Forensics
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
The Replicon Model Replication is controlled by a DNA sequence called a replicator that binds an “Initiator” protein. The initiator protein regulates replication of the DNA DNA synthesis actually begins at the origin, which is usually within or adjacent to the replicator.
Image of page 2
The Roles of the Initiator Initiator binds DNA. Initiator unwinds (a little) DNA at the origin. Initiator recruits other replication proteins.
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Genetic identification of replicators
Image of page 4
The Eukaryotic Cell Cycle The major events of cell division, DNA replication and chromosome segregation, are separated in time, into cell cycle phases. During the Gap phases (G1 and G2) checkpoints ensure that all is well with the DNA and segregation machinery, before allowing DNA replication (S phase) and mitosis (M) to commence.
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern