4 transformation week1 Friday

4 transformation week1 Friday - Lab #4 Transformation week...

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Lab #4 Transformation week #1 Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang Office hours: T/Th 11:00am-12:00 zhengz@usc.edu
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Quiz 1. draw a typical vector, and mark the 3 essential parts. 2. What salt do we use to induce “competency” during heat shock?
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Objectives to understand how plasmid vector is used in molecular biology. to transform DH5 α E. coli cells with 2 types of plasmid DNA Today’s lab will continue for 4 weeks. Week 1 (Lab #4, part I, pre-lab and quiz only) transform plasmids into E. coli cells Week 2 (Lab #4, part II, post-lab only) calculate transformation efficiency inoculate transformed colonies for plasmid propagation Week 3 (Lab #5) isolate transformed plasmid restriction enzyme digest isolated plasmid Week 4 (Lab #6) confirm plasmid identity by restriction mapping
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Plasmids Electron Micrograph of a Ruptured E. coli Cell http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html
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http://www.aliciapatterson.org/APF2202/Sachs/Sachs.html Scanning Electron Microscope 3D photo of E. coli
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4 transformation week1 Friday - Lab #4 Transformation week...

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