2 PCR from ZZ Friday

2 PCR from ZZ Friday - Molecular Biology Lab 2 DNA...

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Molecular Biology Lab 2 DNA Profiling – Polymerase Chain Reaction Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang Office hours: T/Th 11:00am-12:00 zhengz@usc.edu
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https://sites.google.com/a/luther.edu/genetics/students/leah-walters/pcr-amplification Techniques to learn: DNA purification and PCR
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Collect cheek cells 1. Each student needs to collect one sample (no food or drink 30 min prior to sampling). 2. Scrape each cheek 15 times with a Omni swab. 3. Air dry for a minimum of 30 minutes (ends up and label) http://botit.botany.wisc.edu/images/130/Plant_Cell/Animal_cells/cheek_cells.php?highres=true
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Objectives: to determine genotype for Alu insertion and perform Hardy-Weinberg analysis of class result This lab will continue next week. This week (Lab #2) isolate DNA from your cheek cells PCR amplify the Alu sequence from isolated DNA Next week (Lab #3) run DNA agarose gel electrophoresis of the amplified Alu sequence determine genotype of Alu insertion perform Hardy-Weinberg analysis of class result
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2 PCR from ZZ Friday - Molecular Biology Lab 2 DNA...

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