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friday Lab__7_SDS-PAGE

friday Lab__7_SDS-PAGE - Lab#7 SDS-PAGE Electrophoresis of...

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Lab #7 SDS-PAGE Electrophoresis of Proteins Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang Office hours: T/Th 11:00am-12:00 zhengz @ usc . edu
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Today’s lab is related to next week’s lab. This week’s Objectives : to run SDS-PAGE electrophoresis of various fish proteins to calculate the relative mobility of 5 proteins and determine their molecular weights to transfer proteins from the SDS-PAGE gel to a nitrocellulose membrane for Western Blot Next week’s Objective : use Western Blot to identify myosin, a highly conserved protein.
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Actin and Myosin Actin (MW 42 kDa) Muscle fiber Myosin ( heavy chain, MW 210 kDa) (light chain, MW 15-25 kDa) ATP Campell, NA & Reece, JB. Biology , Pearson Education, Inc. San Franscisco, 7th Ed. Fig. 49.30
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Assembling the Module Assembling the Module 1) Use a razor to cut the tape along the 1) Use a razor to cut the tape along the bottom of the gel and pull off the bottom of the gel and pull off the plastic strip.
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