Lab _1-Spectroscopy friday

Lab _1-Spectroscopy friday - Molecular Biology Lab 1...

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Molecular Biology Lab 1 Spectrophotometric Analysis of Nucleic Acids Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang Office hours: T/Th 11:00am-12:00 zhengz@usc.edu If You’re Early and Want to Get a Head Start 1) Turn on your spectrophotometer. 2) Label Tubes (page 11).
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Overview Today you will learn how to: 1) Calculate and perform dilutions. 2) Operate a spectrophotometer. 3) Quantify the concentration of nucleic acids. 4) Determine the purity of nucleic acids.
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DNA Polynucleotide Chain 2-5 g. 2-5 Fig. 2-8 Double-stranded DNA Single-stranded DNA
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Nucleic Acid Bases DNA absorbs UV light at 253 – 271 nm, and absorbs best at 260 nm. Purines Pyrimidines
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Electromagnetic Spectrum
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Campell, NA & Reece, JB. Biology , Pearson Education, Inc. San Franscisco, 7th Ed. Light source, a lamp Tungsten for visible Deuterium for UV Slit controls intensity Light beam Sample solution Monochromator Refracting prism or diffraction gratings Photoelectric tube Transmittance is read and usually converted to absorbance Fig. 10.8
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Lab _1-Spectroscopy friday - Molecular Biology Lab 1...

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