Lab _8 Western friday

Lab_8 Western - Lab#8 Western Blot of Proteins(last lab Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang [email protected] End of Semester

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Unformatted text preview: Lab #8 Western Blot of Proteins (last lab!!) Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang [email protected] End of Semester evaluations Make sure you have the following forms: 3 General Teaching Evaluation Form (Pink) 1 for Prof. Xuelin Wu 1 for Prof. Oscar Aparicio 1 for Prof. Robert Baker 4 Open-end Comment (Blue) same as above + 1 for TA 1 Teaching Assistant Evaluation Form B (Pink) Total forms: 8 • TAs should not handle completed evaluations. • A designated student should submitted the completed evaluations to ZHS 357. Objectives: • To understand the concept of Western Blot. • To detect the protein of interest Antibody Structure • consists of four chains • two heavy chains (blue) • two light chains (pink) Fc : animal-specific Fab: antigen binding fragment Polyclonal and monoclonal antibodies Polyclonal: • recognizes several epitopes on an antigen • heterogeneous Monoclonal: • recognizes one epitope on an antigen....
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This note was uploaded on 12/01/2009 for the course BISC 13019 taught by Professor Aparicio during the Spring '09 term at USC.

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Lab_8 Western - Lab#8 Western Blot of Proteins(last lab Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang [email protected] End of Semester

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