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Lab _8 Western friday

Lab _8 Western friday - Lab#8 Western Blot of Proteins(last...

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Lab #8 Western Blot of Proteins (last lab!!) Section 1332 7 Friday 1:00pm-4:00pm ZHS 359 Zheng Zhang [email protected]
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End of Semester evaluations Make sure you have the following forms: 3 General Teaching Evaluation Form (Pink) 1 for Prof. Xuelin Wu 1 for Prof. Oscar Aparicio 1 for Prof. Robert Baker 4 Open-end Comment (Blue) same as above + 1 for TA 1 Teaching Assistant Evaluation Form B (Pink) Total forms: 8 TAs should not handle completed evaluations. A designated student should submitted the completed evaluations to ZHS 357.
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Objectives: To understand the concept of Western Blot. To detect the protein of interest
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Antibody Structure consists of four chains two heavy chains (blue) two light chains (pink) Fc : animal-specific Fab: antigen binding fragment
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Polyclonal and monoclonal antibodies Polyclonal: recognizes several epitopes on an antigen heterogeneous Monoclonal: recognizes one epitope on an antigen.
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