Lab 1_ Algae and Fungi

Lab 1_ Algae and Fungi - Purpose of this lab Lab 1 Algae...

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1 Lab 1 Algae and Fungi Purpose of this lab • Become familiar with the characteristics of the algal groups Chlorophyta, Phaeophyta, and Rhodophyta Understand the technique and theory behind thin • Understand the technique and theory behind thin- layer chromatography • Become familiar with the characteristics of the fungal divisions Zygomycota, Basidiomycota, and Ascomycota • Understand the symbiotic relationship in lichens Note: the group of unicellular and simple multi-cellular eukaryotes informally known as “ protists ” is presently undergoing reclassification, as more becomes known about them. Protists protists Changing Views of Algal Taxonomy The term “ algae ” refers to relatively simple, eukaryotic, aquatic photoautotrophs. As with other protists, their taxonomic affinities are being re-evaluated. Continued Changing Views of Algal Taxonomy In today’s lab, we will look at 3 algal groups: Chlorophyta (green algae) Phaeophyta (brown algae) Rhodophyta (red algae) Chlorophyta : Green Algae Of the three groups, green algae ( Chlorophyta ) are thought to be most closely related to plants, in part because both green algae and plants contain the photosynthetic pigments chlorophyll a and chlorophyll b .
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2 Chlorophyta : Green Algae Green algae include unicellular, colonial, and multicellular forms. They are found in freshwater, brackish, and saltwater habitats. A number of species of large marine algae (generally called “seaweeds”) are found in Hawaiian waters: Dictyosphaeria cavernosa Halimeda opuntia Codium edule Phaeophyta : Brown Algae Brown algae contain the photosynthetic pigments chlorophyll a and chlorophyll c , as well as accessory carotenoid pigments. They are all multicellular forms, and most occupy marine environments. Phaeophyta : Brown Algae The Hawaiian nearshore marine environment contains a number of brown algal species: Padina japonica Sargassum polyphyllum Turbinaria ornata Rhodophyta : Red Algae Red algae contain chlorophyll a , as well as carotenoids and phycobilins. They are mostly multicellular, marine forms. Red algae are abundant in warm, tropical coastal waters. Rhodophyta : Red Algae The red pigment phycoerythrin (a phycobilin) is sometimes masked by other photosynthetic pigments, so red algae do not always appear red. Hypnea chordacea Asparagopsis taxiformis Ahnfeltia concinna Hawaiian Marine Algae A number of Hawaiian marine algae representing green , brown , and red algae will be on display in the lab today. Be sure to take a look at them, and see if you can identify them.
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3 Thin-layer Chromatography In today’s lab you will be separating the pigments in green , brown , and red algae using a technique called “thin-layer chromatography”, or TLC. In this technique, pigments are separated based on their differential solubility in water and a solvent. The pigments are first extracted
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Lab 1_ Algae and Fungi - Purpose of this lab Lab 1 Algae...

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