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Unformatted text preview: Quality | Selection | Performance | Results Cancer Development Endocrinology Immunology Neuroscience Proteases Stem Cells R&D Systems Cell Biology Focus spring | 2006 Please visit our website @ www.RnDSystems.com for product information and past issues of the Focus Newsletter: Cancer, Neuroscience, Immunology, and more. Inside page 2 Signal Transduction Intracellular Kinases page 3 BMP & GDF Signaling page 4 Extracellular Matrix page 5 Post-translational Modifications page 6 Membrane Sheddases & Their Targets page 7 Adipocytokines page 8 Parameter Kits Small Molecule Assays www.RnDSystems.com Cell Biology FOCUS Signal Transduction: Intracellular Kinases Kinases are integral parts of signal transduction cascades and they regulate virtually all cellular functions. Therefore, it is of critical importance to have quality tools that make it easy to study kinases and their activities. R&D Systems offers a range of tools to study intracellular kinases. These include a wide selection of total and phospho-specific antibodies, and assay kits designed to detect the levels of total and phospho- kinases and their activities. In addition, R&D Systems Proteome Profiler Antibody Arrays allow researchers to simultaneously detect the phosphorylation of a range of kinases in a single sample. To find out more about R&D Systems kinase-related tools, as well as reagents to study receptor tyrosine kinases, phosphatases, transcription factors, and other intracellular signaling molecules, please visit our website at: www.RnDSystems.com/go/SignalTransduction Intracellular Kinase-related Products ANALYTE TOTAL ANTIBODIES PHOSPHO ANTIBODIES TOTAL ELISA PHOSPHO ELISA PHOSPHO ARRAY* Akt (pan) H M R H M H M R H Akt1 H M R H M R H Akt2 H M R H Akt3 H M R H AMPK a 1 H M R H AMPK a 2 H M R H AMPK b 1 H M R ATM H M R H Blk H M R CAM Kinase II Ms CDC2 H M R Chk1 H H M R Chk2 H M R H H ERK1 H M R H M R H H M R H ERK2 H M R H M R H M R H ERK5/BMK1 H M GSK-3 a H GSK-3 a / b H H M R H M R H GSK-3 b H M R H M R H IKK g H M R JNK (pan) H M R H M R H M R H M R H JNK1 H M R H M R H JNK2 H M R H M R H M R H JNK3 H ANALYTE TOTAL ANTIBODIES PHOSPHO ANTIBODIES TOTAL ELISA PHOSPHO ELISA PHOSPHO ARRAY MEK1 H M R H M MEK2 H M R H M MKK3 H M R MKK6 H M R MSK1 H M H MSK2 H M H M H p38 (pan) H M R p38 a H M R H M R H M R H p38 b H p38 d H H H H p38 g H M R H M R H M H p70 S6 Kinase H M R H M R H M R H M R H PDK-1 H PI 3-Kinase p110 H PKR H RSK (pan) H M R H M R H M R RSK1 H M R H M R H RSK2 H M R H RSK3 H M Src H WNK1 M R TOR H M R H H Key: H Human M Mouse Ms Multi-Species R Rat Figure 1. R&D Systems Proteome Profiler MAPK Array (Catalog # ARY002) can simultaneously detect the phosphorylation of 19 different kinases, avoiding time- consuming IP/Western blots. MCF-7 cells were either untreated or treated with 100 ng/mL of human IGF-1 (Catalog # 291-G1) for 1 hour and the lysates examined for kinase phosphorylation. Highlighted are the kinases exhibiting significant phosphorylation in this assay. exhibiting significant phosphorylation in this assay....
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