PS5 - SashaVaziri PCB3063 ProblemSet5 1)

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Sasha Vaziri  PCB 3063 Problem Set 5 1) It’s in alphabetical order and the streptomycin resistance  would be before tetracycline resistance, even though the  chromosome is circular it is transferred linearly through  the pili. Also HFR rarely transfer the whole chromosome and  their pili are unstable. Also the insertion sequence must  be located closer to the streptomycin resistance gene than  tetracycline resistance.  2) Lawn is infected by  r- h+  phage only.  i. Big and cloudy b.  Lawn is infected by  r- h-  phage only. i. Big and Clear  c. Lawn is infected by both  r- h+  and  r- h-  phage. We have two sets or recombinants  r-h- and r-h+ but since h- can infect both types of  strains I believe we will see r-h- big and clear  plaques.  3) The ability to conjugate is determined by the F plasmid  When an F+ cell meets an F- cell, the sex pilli hold them  together, and a copy of the F plasmid is transferred from 
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PS5 - SashaVaziri PCB3063 ProblemSet5 1)

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