Chapter1 supplemental

Chapter1 supplemental - Ligand-receptor interactions...

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Ligand-receptor interactions Ligand: drug or endogenous molecule that can associate with a receptor, transporter, enzyme, or any site of interest Radioligand: radioactively labeled drug. Measuring rate and extent of binding provides information on the number of binding sites, and their affinity and accessibility for various drugs. There are three types of protocols, equilibrium binding, competitive binding, and kinetic study. http://www.curvefit.com
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Saturation binding experiment Analyzes the relationship between the equilibrium binding and ligand concentration to determine the number of sites, Bmax , and the ligand affinity, Kd.
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Procedures “Grind and bind” -uses tissue homogenates Add different concs of [L] to replicate aliquots Add “competitor” ligand in large excess for non- specific binding samples (Specific=total-NSB) Let come to equilibrium, filter, count bound. In situ autoradiography: Mount (brain) sections
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Chapter1 supplemental - Ligand-receptor interactions...

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