Chapter 16 outline - Frederick Griffith uncovered genetic...

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Frederick Griffith uncovered genetic role of DNA Transformation- change in genotype and phenotype due to assimilation of external DNA by a cell Pathogenicity was inherited by all descendants of transformed bacteria Oswald Avery determined DNA was the transforming agent
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1. Incorporate radioactive sulfur on the protein surface of viruses 3. Mix bacteria and viruses in blender and centrifuge 4. Bacteria forms a pellet on bottom and viruses remain in the liquid 2. Viruses infect bacteria
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Same process as before except radioactive phosphorus was incorporated into phage DNA Experiment showed DNA is phage’s genetic material
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A pairs with T G pairs with C Erwin Chargaff found that the four nitrogenous bases were present in specific ratios in all organisms. Could not explain his discovery A= 30.9% G= 19.9% T= 29.4% C=19.8%
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Watson saw an image of DNA produced by x-ray crystallography and deduced DNA has a helical shape.
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This note was uploaded on 12/02/2009 for the course SCI 90210 taught by Professor Durkka during the Spring '09 term at École Normale Supérieure.

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Chapter 16 outline - Frederick Griffith uncovered genetic...

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