lecture_21

lecture_21 - Lecture19cont Simplesystems: and/orinducers...

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Lecture 19 cont.
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4 general regulatory systems Simple systems : metabolites acting as co repressors and/or inducers Global Control : small molecules as second messengers Two component regulator systems : a highly conserved group of signal transduction pathways that involve a protein phosphorylation system. Multiple Sigma Factors : Sequential activation of sigma factors involved in a developmental pathway.
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Complex signal transduction pathway: Two component regulatory systems: Composed of two families of homologous domains that communicate with each other using a conserved mechanism involving phosphorylation.
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In its simplest form it contains two proteins: A sensor protein that receive the information and a response regulator that bring about a change in activity. Sensor Domain Kinase Domain Receiver Domain Output Domain Signature domains
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Sensor Kinase Family Members of this family have a very diverse N terminal domain and a highly conserved C terminal transmitter domain. Input or Sensor domain recognizes a specific signal and is thought to affect the biochemical properties of the C terminal Kinase domain. This domain can receive information from the periplasm, in which case it has a membrane spanning domain such as EnvZ or PhoR , or it can receive information from the cytoplasm such as NtrB or KinA .
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Kinase structure 4 conserved regions within the kinase domain: H Box contains the His residue that is phosphorylated phosphoramidate N Box F Box believed to be involved in Mg binding DxG motif G Box Nucleotide binding domain H N G1 F G2 Phosphorylation site
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SK activities The domain has autokinase activity that attaches the phosphoryl group from ATP to the histidine residue forming a phospoamidate bond, this is a high energy bond and easily removed The domain has phospho transferase activity that transfers the phosphoryl group to the aspartate residue
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This note was uploaded on 12/02/2009 for the course MIC 140 taught by Professor Meeks,singer during the Spring '09 term at UC Davis.

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lecture_21 - Lecture19cont Simplesystems: and/orinducers...

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