Midterm II Fall 09 - Answer Key.doc

Midterm II Fall 09 - Answer Key.doc - RNA Shine Delgarno...

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RNA Shine Delgarno Transcription DNA TATA -10 Transcription DNA TTGACAT -35 Transcription DNA Start Site +1 Transcription RNA Terminator BIS101-01, Fall 2009 Dr. D. J. Kliebenstein, Instructor Midterm Exam II Please print your name and ID number on page 2 and the rest of the exam. Do not print your ID number on the first page. The exam has 10 questions and a total of 120 points For full credit, show all of your work. AUTHORIZATION FOR PUBLIC DISTRIBUTION OF GRADED PAPERS OR EXAMINATIONS I,_________________________________, Authorize the University to publicly distribute my graded exam (e.g., handed out in class or left in an exam bin) for BIS101-01. If I do not sign, then I must see the instructor to pick up my exam. Signed:_______________________________________ Dated:__________________________________
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1. __________ 2. __________ 3. __________ 4. __________ 5. __________ 6. __________ 7. __________ 8. __________ 9. __________ 10. __________ Total _________
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1. (18 points) Complete the following Table, assuming that the DNA is written with the top left being a 5’ end and that the columns represent transcriptional and translational alignments. Further, reading is from left to right. For DNA, use ACTG or N where N represents any possible nucleotide. A T C A T G A G T C C A DNA Double Helix T A G T A C T C A G G T A U C A U G A G U C C A mRNA U A G U A C U C A G G U tRNA Anticodon ILE MET SER PRO Amino Acids Which of the above rows is the template strand? The first DNA strand is the coding strand. The bottom table is typically thought of as describing the tRNA’s, are there any codons that do not correspond to tRNA’s? If so, what are they and what do they do? The UAA, UAG and UGA codons do not represent tRNA’s and they cause translation to stop. 2. We discussed two types of donor DNA that can be used to make libraries to find the DNA for a gene. (8 pts)
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a. What are these two types of DNA and where did they come from? These are genomic DNA that was isolated from the genome and cDNA which was made from RNA b. Which type of library would you use for functional complementation when studying a prokaryote? Either library would work.
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This note was uploaded on 12/03/2009 for the course ENT 100 taught by Professor Kimsey,r during the Fall '08 term at UC Davis.

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Midterm II Fall 09 - Answer Key.doc - RNA Shine Delgarno...

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