Midterm II Winter 06 - Answer Key

Midterm II Winter 06 - Answer Key - BIS101-01, Spring 2004...

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BIS101-01, Spring 2004 Dr. D. J. Kliebenstein, Instructors Midterm Exam II Please print your name and ID number on each page of the exam. The exam has 12 questions and a total of 120 points. For full credit, show all of your work. AUTHORIZATION FOR PUBLIC DISTRIBUTION OF GRADED PAPERS OR EXAMINATIONS I, _______________________________, student ID No.__________________________________: authorize the University to publicly distribute my graded exam (e.g., handed out in class or left in an exam bin) for BIS101-01. If I do not sign, then I must see the instructor to pick up my exam. Signed: ________________________________ Dated: _______________ 1
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1 __________ 2 __________ 3 __________ 4 __________ 5 __________ 6 __________ 7 __________ 8 __________ 9 __________ 10 __________ 11 __________ 12 __________ Total _________ 2
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1. (7 points) Are the following components involved in DNA replication? Answer Yes or No. RNA dependent DNA Polymerase _____ No_or Yes _______ DNA Polymerase _____ Yes ________ Helicase ______ Yes _______ Reverse Transcriptase ______ No ______ Topoisomerase ______ Yes _______ Deoxyribonucleic Acid ______ Yes _______ Ribonucleic Acid ______ Yes _______ 2. (12 points) In lecture, I listed three ways that post-translational regulation can occur. Describe these three potential regulatory mechanisms. The quaternary structure can be regulated by reactions such as the addition of a phosphate or a protease that cleaves a section off. The location of the proteins can be such that it can be kept our of the compartment in which it is activated. Proteolysis through the action of ubiquitin and the proteosome can tag proteins for degredation. b. These mechanisms control post-translational regulation. What other levels of regulation can post- translational regulation potentially impact (Only 3 of the 12 points)? All other levels involve proteins at some level and as such, all levels could be impacted. 3
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3. (14 points) Translation Involves Ribosomes, t-RNA’s and mRNA. Shown below is a rough drawing of a ribosome that has already bound mRNA. Translation has already been initiated and is currently in the elongation stage. Label the appropriate regions of the Ribosome. At the start of a lecture, I described four steps to translation elongation. Briefly describe the major change that occurs at each of the five steps. Also, make sure to state where the various t-RNA’s (empty, aminoacyl and peptidyl) are located within the ribosome. Step 1) The peptidyl tRNA is in the P site and a new Aminoacyl tRNA enters the A site. ( 2 points) Step 2) A new peptide bond is formed and the peptide chain is transfered to the A site. An empty tRNA is in the P site and the new peptidyl tRNA is in the A site. (3 points)
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This note was uploaded on 12/03/2009 for the course ENT 100 taught by Professor Kimsey,r during the Fall '08 term at UC Davis.

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Midterm II Winter 06 - Answer Key - BIS101-01, Spring 2004...

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