Analysis 6 - Analysis 6: Chloroplast Isolation 1....

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Unformatted text preview: Analysis 6: Chloroplast Isolation 1. Chlorophyll concentration 2. Chlorophyll concentration 3. Chl a and chl b a. Chl a b. Chl b 4. Chl b 5. Ratio 6. Height of solvent from the origin is 135mm Pigment Height (mm) Rf Beta-carotene 105 .777 Chlorophyll a 95 .704 Chlorophyll b 90 .666 During the Chromotography, the most soluble and polar molecule with move the fastest. Beta-carotene moved the fastest by covering 105mm of the 135mm and then it was followed by chlorophylla and that was followed by chlorophyllb. 7. Chlorophyll a (blue) 425nm, chlorophyll a (red) 665nm Chlorophyll b (Blue) 460nm, chlorophyll b (red) 665nm Beta-carotene (blue) 450nm, bête-carotene (red) 640nm The data that was generated off of the chlorophyll graph is as follows. It makes sense to have a large rise in the 420-480nm section of the wavelength because that is the blue being absorbed. I t also makes sense to have a rise is the 640-670nm section of the wavelengths because that is the red being absorbed. I t also makes sense that...
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This note was uploaded on 12/04/2009 for the course BIO 50100 taught by Professor Brand during the Spring '09 term at University of Texas.

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Analysis 6 - Analysis 6: Chloroplast Isolation 1....

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