Analysis 8 - Marta Gebregziabher Analysis 8: Protein...

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Marta Gebregziabher Analysis 8: Protein Electrophoresis 1. Glycerol is included as a component of the Gel Loading Buffer because it is included in all of the samples to prevent them from floating up, and since its dense it keeps them down at the gel. 2. Water molecules have a partial positive and negative charge because of the high electronegetivity of the oxygen atom. The hydrogen’s are partially positive and the oxygen’s are partially negative. When power is applied to the gel the cathode or the part that attracts the cations is at the top and the anode or the part that attracts the anions is at the bottom. So since hydrogen had a partial positive charge, it will be facing the cathode and since oxygen has a partial negative charge it will be facing the anode. 3. All the water molecules are not aligned in the same orientation because; the gel contains more solutions than water.
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This note was uploaded on 12/04/2009 for the course BIO 50100 taught by Professor Brand during the Spring '09 term at University of Texas at Austin.

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Analysis 8 - Marta Gebregziabher Analysis 8: Protein...

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