analysis 9 - Marta Gebregziabher Analysis 9: Protein...

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Marta Gebregziabher Analysis 9: Protein Analysis 1. Gel Half 1 has significantly more bands than does Gel Half 2. The bands were also significantly darker. Both half’s had bands in the same 1, 4 and 5 lanes but there were more bands in gel half 1 than gel half 2. The bands indicate that there were more protein samples in gel half 1 than in gel half 2 because the proteins from gel 2 were electrotransfered. The electrotransfer of gel half 2 was not successful because of many reasons. One of the reasons could be the fact that the electrotransfer was not long enough for the proteins to transfer to the membrane. Another factor could be that the stacks that were socked in the cathode solution and the stacks that were socked in the anode solution could have been touching, compromising the whole process of transfer. 2. All the liver and tripe Proteins are detected by the Coomassie Blue dye. Using the primary seconday detection system we, are able to visualize the albumin protein. 3.
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This note was uploaded on 12/04/2009 for the course BIO 50100 taught by Professor Brand during the Spring '09 term at University of Texas at Austin.

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analysis 9 - Marta Gebregziabher Analysis 9: Protein...

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