Biology1510_Shetty_FallUnknownYear_Exam2_1_[1]

# Biology1510_Shetty_FallUnknownYear_Exam2_1_[1] - ,~,p BIG...

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// ,~# "",p.-'" <0.,-- , ..,:-1) 'I. BIG 1510 Exam 2 Version: III GTA: Ameet S. Shetty /~~. / \ I t4'7 . i \/v ) \~// , . , """'------- Stuot..- Date: Deceh. '_"wnin A. Use the two graphs below to answer the following four questions. Each graph plots the reaction rate of Enzyme A (thick line) and Enzyme B (thin line) against either pH or temperature. 4 R c. 3 .i 0 2 R e 2 6 pH 10 12 4 4 R c 3 0 2 n R a I 14. 30 40 60 50 10 20 Temperature ~~ic~.lwO enzymes operates over a narrower pH range? (l pt) ~' 8 1. ~:umPHforEnzyrneA? (Ip!) 2. 3. Which of the two enzymes shows greater activity when placed in a refrigerator? (l Pi ft Which data set below best fits Enzyme A? (1 pt) 4. ~/ ~-).~ 5e-+ r:;c . Data Set pH=3 pH=5 pH=9 . oH=II 5°C 20°C 30°C 40°C X 1.5 3.2.5----' 1.5 0.75 1.5 3.25 2.0 1.0 Y 1.0 -----------3.0 2.5 0.75 1.5 4.0 2.5 0.75 . Z .;() 0.0 2.0 2.25 0.0 1.25 3.0 1.0

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~-"y , n- --.-.. .' , .. ~-- 1'-' I B. In the space provided, draw a graph that represents the substrate concentration data shown in the table below. Draw a vertical line to indicate that point at which the enzyme is saturated. (1 pt) ,.C'\ r R It w e i' 11 \ : ' I ~[ ./ j. ,...~:' .r ., ","'\ ,.(fo- 01'-' '1<"" ~ 0f1- ,j ----- .... 3 R ~' / /~ . / I a t 10 20 30 40 so 60 70 80 . 90 100 Substrate Couceutratiou (( ~ \ n-f CO? 'i}1'-"e c. You have set up two vials to measure respiration in peas in cold and warm environment.s. You placed 15 sprouts into each of two vials also containing a cotton ball and KOH. You then placed a graduated 1 ml pipette on the end of each vial. You placed one in a warm environment and the other in a cold environment. . a.. After ~hat direction did the water in the warm environment pipette . ~ove1~c.r:z? ~)v-l.$.tv \)Je>,.rJ' Sp-nl.-t-b . hu.o(,ull CA.$ ,P71?~P ~)~c$' 6'2-, ~. .~~ ;<; u",,-9,r,t~ 1,'}- )~t'H . So ~6I..$ tyt_h~ del.y~1a,-,~t~ I"".,';""lt. tl.t Ve~s.L) . ... ~ .~IJ I{~ ~"'>--t!w~ llA7-0, .J--ot.JiJvrJs 5IYDL-t.ts. b. What dIrectIOn dId the water m the cold enVIronment pipette move? Why? (1 pt) "\ Lt wet -tlX rJ.1I if) Yttr'f- v,",-" vt- 1'V'-vV( t~- . T.J: Hio t.vI' , I, --0 \r/!.. fu ...... (\- l.>,IJ\ 1 \ ---o-v-f- .~ !l<-H'.-Y d ~ .b c C~\..~t I'V\.. Co \ ~ l--vvV-1 Y1)""" ~- iW.. 'L"'" 2- ~ """'5 ) \)) k,' c..-k C\"vL. '",- ~ (I e...oU ;'. ; \..- L~ I\ V1. \ ()t y Y.f!~ (> hret -H~'~) tu,' ' . 1 cl v-.tt oh-t."t . f4-vJ S;,:} I (" . dD e.,:; n~' . t:r'i)p~I/(j - ,;.tJ ~\$ Pyt'J!!:. u'\--t. ,,~f ""'-'-Ot ! ~ 'SCi v---e . ,' ~ v-t»-t-I. (L 10% 20% 30% 40% 50% 60% 70% 80% 0.0 1.25 1.75 2.25 2.5 2.5 2.5 2.5
".' """"'Ii" : " ~ / . . I ',; w ;" =------.. D. You and your lab partners added yeast and one of the test solutions to fill a small test tube. You then inverted a large tube over each small tube and inverted the two tubes. After taking initial measurements, all 6 were then placed in a warm water bath in hopes that the yeast would respire. You remeasured each tube at 30, 60 and 90 minutes and made the appropriate calculations to complete the data table below. Use this data table to answer the questions that follow. 1. In which solution(s) did the yeast respire most over the entire course of the experiment? (1 pt) -------- ' ~\ \,\to~~ 2. In which solution(s) did the yeast respire most over anyone 30 minute time period? (1 pt) ~ E. You and your lab partners added yeast and one of the combinations oftest solutions to fill a small test tube. You then inverted a large tube over each small tube and inverted the two tubes. After taking initial measurements, all 5 were then placed in a warm water bath in hopes that the yeast would respire. You remeasured each tube at 30, 60 and 90 minutes and made the appropriate calculations to complete the data table below Use that data table to answer the question that foil 1. C:°mparing tubes 3 and 4, what specificall

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