341lecture29nov20sakai

341lecture29nov20sakai - Cell Biology 341 Last lecture:...

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Cell Biology 341 Friday, November 20, 2009 Today: conclude chapter 14 • ATP synthase uses the energy stored in the proton gradient Begin chapter 15: Last lecture: • Mitochondria contain two membranes separated by the intermembrane space • The inner compartment is the matrix • During chemiosmosis, the complexes of the electron transport chain create an electrochemical gradient of H + across the inner membran mechanisms of cell communication • Answers to chapter 13 questions are on Sakai inner membrane • The gradient is used to drive ATP synthesis • Electrons are transferred to carriers with increasing affinity, from the NADH dehydrogenase complex → Q → cytochrome b-c 1 complex → cytochrome c → cytochrome oxidase complex → O 2 • 10 H + are pumped into the intermembrane space for each NADH donating 2 electrons
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Cytochrome c carries electrons between the b-c 1 complex and the cytochrome oxidase complex • Cytochrome c is a small protein found in the intermembrane space • When it binds the b-c 1 complex and then the cytochrome oxidase complex, it becomes a peripheral protein on the outer face of the inner membrane • The cytochrome b-c 1 complex and the cytochrome oxidase complex contain other bound cytochromes, which have increasingly higher affinity for e - • The iron atoms in the heme groups of the cytochromes have high affinity for electrons • Fe 3+ + e - → Fe 2+ Figure 14-22 the heme group attached to cytochrome c
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The cytochrome oxidase complex contains both copper and heme iron
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This note was uploaded on 12/05/2009 for the course BIO 341 taught by Professor Noris during the Fall '09 term at Rhode Island.

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341lecture29nov20sakai - Cell Biology 341 Last lecture:...

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