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341lecture07sept23sakai

341lecture07sept23sakai - Cell Biology 341 Last lecture...

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Cell Biology 341 Weds., Sept. 23, 2009 Today: The structure of chromosomal DNA DNA is packaged into nucleosomes higher levels of chromatin packaging Go to Molviz.org and view the DNA tutorial See videos 4.1 (hydrophobic forces → basepair stacking), 4.3, 4.4 and 4.5 on your DVD Answers to chapters 3 and 4 questions and an old exam have been posted on Sakai Last lecture: The active site stabilizes the transition state of the reaction Examples of prosthetic groups 3 ways proteins may be rapidly and reversibly regulated: allosteric sites, phosphorylation or GTP binding Chapter 4: DNA structure Nucleotides are linked by phosphodiester bonds H-bonding, antiparallel strands, helical structure, major and minor grooves DNA is stabilized by hydrophobic forces, hydrogen bonding and van der Waals forces
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Implications of DNA structure If we know the sequence of bases in one strand, we can predict the sequence of the complementary strand 5'-GATCAAGCT-3' 3'-CTAGTTCGA-5'
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