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BIO172W08Lec21CT - Biology 172 L e c t u r e 2 1 E u k a r...

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1 Lecture 21: Eukaryotic Gene Regulation I March 5, 2008 Today s Outline 1. DNA binding proteins – proks and euks 2. Overview of euk gene expression 3. Chromatin organization Announcements Make-up Policy on Quizzes: 1) excused absence, 2) make up quiz within one week, 3) go to ANY GSI’s office hours Biology 172 Review of lac Operon repressor lac tose CAP-cAMP Binding sites in the lac operon Operator Many nucleotide sequences that bind proteins have two-fold rotational (dyad) symmetry Lac Repressor protein binds O 1 and one of the other two sites O 2 or O 3 5’ 3’ 3’ 5’ Helix-Turn-Helix Motifs in DNA-Binding Proteins Secondary & Tertiary Protein Structure Quaternary Structure of the Lac Repressor Protein to the lac operon Operator DNA forms loops that bring protein binding sites physically closer together Each half of the symmetrical Operator sequence is bound by a helix- turn-helix DNA binding motif in one subunit of the Repressor protein
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2 Conformational change when Inducer (lactose) binds Repressor (protein) of the lac operon Active form Inactive form Major Types of DNA Binding Protein Motifs in Eukaryotes Bind to major groove of DNA helix Binding involves many types of intramolecular bonds >> together provide very specific and strong binding interaction Eukaryotic Gene Regulation
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