BIO172W08Lec21CT - Biology 172 Lecture 21: Eukaryotic Gene...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon
Lecture 21: Eukaryotic Gene Regulation I March 5, 2008 Today’s Outline 1. DNA binding proteins – proks and euks 2. Overview of euk gene expression 3. Chromatin organization Announcements Make-up Policy on Quizzes: 1) excused absence, 2) make up quiz within one week, 3) go to ANY GSI’s office hours Biology 172
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Review of lac Operon repressor lac tose CAP-cAMP
Background image of page 2
Binding sites in the lac operon Operator Many nucleotide sequences that bind proteins have two-fold rotational (dyad) symmetry Lac Repressor protein binds O 1 and one of the other two sites O 2 or O 3 5’ 3’ 3’ 5’
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Helix-Turn-Helix Motifs in DNA-Binding Proteins Secondary & Tertiary Protein Structure
Background image of page 4
Quaternary Structure of the Lac Repressor Protein
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
to the lac operon Operator DNA forms loops that bring protein binding sites physically closer together Each half of the symmetrical Operator sequence is bound by a helix- turn-helix DNA binding motif in one subunit of the Repressor protein
Background image of page 6
Conformational change when Inducer (lactose) binds Repressor (protein) of the lac operon Active form Inactive form
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Major Types of DNA Binding Protein Motifs in Eukaryotes Bind to major groove of DNA helix Binding involves many types of intramolecular bonds >> together provide very specific and strong binding interaction
Background image of page 8
Image of page 9
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 20

BIO172W08Lec21CT - Biology 172 Lecture 21: Eukaryotic Gene...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online