AnswerkeyQuiz_4 - Answer key for Quiz #4 If one adds an...

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Answer key for Quiz #4 If one adds an inhibitor of phosphofructokinase (which catalyzes reaction 3) to a cell extract and then adds radioactive glucose, the radioactivity accumulates in Glucose 6-phosphate. Briefly explain why. There are lots of ways to get full credit on this question. All they have to say is that reaction 2 does not go forward due to an increase in Fructose-6- phosphate. Below are some actual answers and the points awarded. 10 pt answers (this answer is not as good as many I saw, but still deserves full credit) When reaction 3 is inhibited, a high concentration of fructose-6-phosphate builds up. This can be seen as a “signal” to stop reaction 2. Since there is an abundance of fructose-6-phosphate, the product of reaction 2, reaction 2 stops. The large amount of fructose 6-phosphate gives the reaction a positive delta g, and the reaction will no longer be spontaneous. Since reaction 1 is powered by ATP, the reaction will continue. This causes a buildup of
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This note was uploaded on 12/06/2009 for the course BIO 172 taught by Professor Clark during the Spring '08 term at University of Michigan.

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AnswerkeyQuiz_4 - Answer key for Quiz #4 If one adds an...

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