Answerkeyquiz_5 - and thus increasing enzyme activity....

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What happens to the following compounds when cyanide is added? Cytochrome C (3pts) Full Credit Answers: All the cytochrome C is reduced All the cytochrome C is saturated with electrons Cytochrome C is reduced (or saturated with electrons) and there is a build up of electrons which cause free radical damage (2pts) Partial Credit: If they state that there will be a build up or excess of electrons that cause free radical formation without mentioning what happens to Cyt. C Phosphofructose Kinase (3pts) Full Credit answers: At the start, high levels of ATP inhibit the enzyme. However, once the electron transport chain is inhibited, ATP levels dramatically decrease and there is an increase in enzyme activity. Enzyme activity is increased (they don’t need to say that at first the activity is low) (1pt) Partial Credit answer If they understand that high levels of ATP decrease enzyme activity, but then don’t say anything about the concentration of ATP decreasing after cyanide addition
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Unformatted text preview: and thus increasing enzyme activity. Pyruvate Dehydrogenase (4pts) Full credit answers That the enzyme is caught between two forces: lower ATP after CN addition will activate it, but high levels of NADH will inhibit it. If they say that the high levels of NADH will inhibit the enzyme and this will promote fermentation, this is a full credit answer. (2pt) partial credit answer If they say that the enzymes activity goes down because of high NADH or NADH and ATP and there is no mention of fermentation. If they say that the enzyme will go down to promote fermentation but dont mention NADH If they say that the enzyme activity will increase due to reduced ATP. (1pt) partial credit answer Poorly worded answers when they say the enzyme goes up or down, but the reason they give incorrect (e.g., high levels of ATP activate) If they do not answer the question of what happens after cyanide is added....
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Answerkeyquiz_5 - and thus increasing enzyme activity....

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