Lecture 11 - Fermentation and Bioprocessing Understand what...

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Fermentation and Bioprocessing Understand what is meant by the term bioprocessing and its purpose. Be able to explain what occurs in upstream processing and how it differs from downstream processing. Understand the factors that impact growth and how these factors are provided for when cells are grown in the laboratory or industrial setting. Be able to explain the phases of a growth curve.
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Fermentation and Bioprocessing Be able to describe the various types of vessels that can be used for growth of cells. Be able to explain the differences in scale that each vessel represents, the problem that such differences create and how these problems are addressed by the various types of growth vessel. Understand how the products produced by cells are harvested. Know the differences in harvesting methods for intracellular and extracellular products. Be able to describe the methods and considerations associated with disruption of cells for the harvesting of intracellular product.
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Bioprocessing Commercial Bioprocessing Raw Materials Products Cells Or Enzymes
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Fermenters (Bioreactors)
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Bioprocessing Upstream Cell culture Scale up Downstream Analysis Purification
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QC / QA Quality Control Monitors upstream and downstream processing Environmental monitoring Quality Assurance Handles paperwork from various divisions in the manufacturing facility
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cGMPs current Good Manufacturing Practices Mandated by the FDA (http://www.fda.gov/cder/dmpq/) Quality Assurance: everyone’s compliant Food, pharmaceuticals, medical devices Holistic approach of regulating the manufacturing and laboratory testing itself Documentation, documentation, documentation!
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Microbial Propogation and Nutrition Size Vs. Numbers
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Binary Fission Generation Time
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Bacterial Growth is Exponential Generation (doubling) time = 20 min 0’ 20’ 40’ 60’ 80’ 100’ 120’ 1 2 4 8 16 32 64 3 Generations per hour After 24 hours 4,722,366,482,869,645,213,696
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Bacterial Growth is Exponential 20 hours
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Growth Curve I Phase I Lag Phase
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Growth Curve II Phase II Exponential or Log Phase
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Growth Curve III Phase III Stationary Phase
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Growth Curve IV Phase IV Death Phase
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Feedstock Raw materials used in bioprocessing Growth media
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Lecture 11 - Fermentation and Bioprocessing Understand what...

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