Lecture 9 - Recombinant DNA Technologies Understand the...

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Recombinant DNA Technologies Understand the reasons for genetically engineering organisms. Be able to explain the steps that undertaken in a genetic engineering project. Be familiar with the various cloning vectors that exist. Understand the limitations of these vectors. Understand the methods for introduction of DNA into cells. Understand how transformed cells are differentiated from non-transformed cells and why such differentiation is necessary. Understand what reporter genes are why these systems are necessary.
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Genetic Engineering “New” biotechnology Mutagenesis Vs. Transgenics
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Mutagenesis
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Transgenics
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Reasons for Transgenics Agricultural
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Reasons for Transgenics Medicine and Research
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Reasons for Transgenics Medicine and Research
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Recombinant DNA Technologies Collection of molecular techniques 1.Development of libraries 1.Genetic engineering
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Genomic Library
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Genomic Library
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Complementary DNA (cDNA) Library
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Overview of Genetic Engineering Select DNA Create DNA construct Introduce DNA construct into host organism Differentiate: Transformed vs. Non-transformed Propagation of clones
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Goals of Genetic Engineering Produce large quantities of a specific DNA Produce large quantities of a protein encoded by specific DNA Modify an organism Temporary: Extrachromosomal Permanent: Integration
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Selecting DNA Insert DNA Foreign DNA Transgene Known DNA sequence vs. Unknown DNA sequence
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Creating a DNA Construct Cloning vector + Insert DNA = DNA construct Restriction Endonucleases DNA Ligase Sometimes……. . not so “pretty”
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Introduce DNA Construct into Host Transformation: molecular biology (Transfection) Bacteria Transformation Conjugation Transduction
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Introduce DNA Construct into Host Transformation: molecular biology (Transfection) Bacteria Transformation
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Introduce DNA Construct into Host Transformation: molecular biology (Transfection) Bacteria Conjugation
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Introduce DNA Construct into Host Transformation: molecular biology (Transfection) Bacteria Transduction
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Transformed vs. Non-Transformed
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This note was uploaded on 12/07/2009 for the course BIOT 101 taught by Professor Don during the Fall '09 term at Ivy Tech Community College.

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Lecture 9 - Recombinant DNA Technologies Understand the...

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