Lecture 10 - DNA Sequencing and the Human Genome Project Be...

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DNA Sequencing and the Human Genome Project Be able to list and explain the benefits of knowing the exact DNA sequence of an organism Be able to explain how dideoxynucleotides are used in the Sanger method of DNA sequencing. Understand why polyacrylamide electrophoresis is important in this process.
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DNA Sequencing and the Human Genome Project Understand how radioactive and fluorescent tags are used in DNA sequencing Be able to interpret a “traditional” sequencing gel, a fluorescent-tagged sequencing gel, and the graphic representation from a high- throughput sequencing run.
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Frederick Sanger Double Nobel Laureate 1.Determined the amino sequence and structure of insulin Tedious Time consuming 1975 1958
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Frederick Sanger Double Nobel Laureate 2. Dideoxynucleotide sequencing Chain-termination sequencing Cycle sequencing Sequenced the genome of bacteriophage Φ -X174 Analyze genes at the nucleotide level 1975 1980
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Dideoxy Sequencing cDNA Find open reading frame (coding sequence) Determine amino acid sequence
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This note was uploaded on 12/07/2009 for the course BIOT 101 taught by Professor Don during the Fall '09 term at Ivy Tech Community College.

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Lecture 10 - DNA Sequencing and the Human Genome Project Be...

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