Lecture Handout 11 - BIOT 101 Lecture Handout 11...

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BIOT 101 Lecture Handout 11 Fermentation and Bioprocessing Vocabulary Acidophil Aeration Airlift fermenters Aspect ratio Autoclave Batch fed fermentation Batch fermentation Bead mills Binary fission Bioprocess Bioreactors Bubble column fermenter Catalase Centrifugation Continuous fermentation Current Good Manufacturing Practices Death phase Downcomer Downstream processes Exponential death phase Exponential growth phase Extracellular product Extreme thermophilic organisms Facultative anaerobes Feedstock Fermenters Fetal bovine serum Filtrate Filtration Gamma radiation Generation time Growth curve Growth media Headspace volume Impellar Intracellular product Lag phase Media Mesophilic organisms Obligate aerobes Obligate anaerobes Pellet Pneumatic reactors Propagation Psychrophilic organism Psychrotrophic organisms Quality assurance Quality control Rotor Shake flasks Shear forces Sonication Sparger Spinner flasks Stationary phase Stirred tank reactor Supernatant Superoxide dismutase Thermophilic organisms Upstream processes Working volume Objectives Understand what is meant by the term bioprocessing and its purpose. Be able to explain what occurs in upstream processing and how it differs from downstream processing. Understand the factors that impact growth and how these factors are provided for when cells are grown in the laboratory or industrial setting. Be able to explain the phases of a growth curve. Be able to describe the various types of vessels that can be used for the growth of cells. Be able to explain the differences in scale that each vessel represents, the problem that such differences create and how these problems are addressed by the various types of growth vessel. Understand how the products produced by the cells are harvested. Know the differences in harvesting methods for intracellular extracellular products. Be able to describe the methods and considerations associated with disruption of cells for the harvesting of intracellular product. 1
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BIOT 101 Lecture Handout 11 Fermentation and Bioprocessing I. The term bioprocess refers to the use of cells, or the enzymes from cells, to convert raw materials into a product. Many industrial processes simply mix raw materials with enzymes to synthesize the desired product. Other bioprocesses involve the growth of bacterial or eukaryotic cells in the presence of the raw materials to yield the desired product. We will concentrate on those bioprocesses that rely upon cells to convert the raw materials to a useful product. A. Currently, the most common bioprocesses involve the production of commercial goods like wine, cheese and beer. These processes rely upon yeast and bacteria to convert raw materials into the desired product. Though the species and strains of microbes used in these processes have been selected for centuries, most of them have not been genetically engineered. B.
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Lecture Handout 11 - BIOT 101 Lecture Handout 11...

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