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Molecular Biology BIMM100 sample midterm 2009 Fall

Molecular Biology BIMM100 sample midterm 2009 Fall - 1...

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1 Molecular Biology BIMM100 M ID - TERM EXAM QUESTIONS FROM YEARS GONE BY 1) a) Why is the ratio of adenine to thymine always the same while the ratio of adenine to guanine varies in the DNA of different organisms? b) What aspects of the structure of DNA are essential for its consistant width? 2) Compare and contrast the steps in DNA replication along the leading strand and the lagging strands. 3) Compare and contrast bacterial and eukaryotic mRNAs. 4) A) Why is it necessary to add two primers to carry out a Polymerase Chain Reaction (PCR)? B) What would the product be if only one primer were added? 5) Why is it necessary for a plasmid vector that can be used for molecular cloning in bacteria to carry a gene that can provide drug resistance to its host bacterium? 6) Why are bacterial chromosomes and plasmids circular? 7) What is the chemical basis that accounts for many single base errors in replication? 8) Give 5 components essential for translation of mRNA and briefly describe their functions.
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